Wnt-3a Protein 5036-WN-010/CF from R&D Systems, a Bio-Techne Brand

Product Specs
ItemWnt-3a Protein
CompanyR&D Systems, a Bio-Techne Brand
Price $452.00Supplier Page View Company Product Page
Catalog Number5036-WN-010/CF
SpeciesHuman
FormatLyophilized from a 0.2 µm filtered solution in PBS, EDTA and CHAPS
Source/Expression SystemCHO-derived Recombinant Human Wnt-3a Protein, CF
Size10 ug (also 500 ug)
Molecule NameWnt-3a
NCBI Full Gene NameWnt family member 3A
ApplicationsBioactivity
Conjugate/TagUnconjugated
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution
Purity>75%, by SDS-PAGE under reducing conditions and visualized by silver stain

Specifications/Features

Available conjugates: Sizes Available: 10 ug (also 500 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Non-invasive in vivo monitoring of transplanted human intestinal organoids using bioluminescenceSurg Open SciMay 15, 2026Chioma Moneme, Antonio Vinicios Alves da Silva, Prisca C. Obidike, Bryan A. Hogg, Georgia B. Brousseau, Casandra Robinson, Christian Roig-Laboy, José Kleybson de Sousa, Yuwen Zhang, Lily S. Cheng, et al.supplemented with 100 ng/mL Activin A (R&D Systems, #BT-ACTAH-050) and 20 ng/mL rhWnt3a (R&D Systems, #5036-WN-010/CF). Cells were then cultured for an additional 48 h in RPMI 1640 containing 100 ng/mL Activin A, 8 ng/mL

(2) Construction of Large‐Scale Bioengineered Hair Germs and In Vivo TransplantationAdvanced ScienceMarch 5, 2025Yangpeng Chen, Yuhui Hou, Jiejian Chen, Jiaojiao Bai, Lijuan Du, Chen Qiu, Hanzhou Qi, Xuanbei Liu, Junfei HuangLive/Dead reagent (#L3224/C3099) were purchased from Invitrogen (Carlsbad, CA, USA). Recombinant Wnt3a (#5036‐WN‐010/CF) was purchased from R&D Systems, USA. Detailed reagents and materials list is shown in Table S2 (Supporting

(3) Nicotinamide and pyridoxine stimulate muscle stem cell expansion and enhance regenerative capacity during agingJournal of Clinical InvestigationDecember 16, 2024Sara Ancel, Joris Michaud, Eugenia Migliavacca, Charline Jomard, Aurélie Fessard, Pauline Garcia, Sonia Karaz, Sruthi Raja, Guillaume E. Jacot, Thibaut Desgeorges, et al.SC79 (Sigma-Aldrich, SML0749), 8 μM TAK-715 (Sigma-Aldrich, SML0360), 200 ng/mL WNT3A (R&D Systems, 5036-WN-010/CF), or 1 μM MK-2206 (Selleckchem, S1078). Immunocytochemistry and image acquisition were performed as

(4) Nicotinamide and pyridoxine stimulate muscle stem cell expansion and enhance regenerative capacity during agingJ Clin InvestDecember 16, 2024Sara Ancel, Joris Michaud, Eugenia Migliavacca, Charline Jomard, Aurélie Fessard, Pauline Garcia, Sonia Karaz, Sruthi Raja, Guillaume E. Jacot, Thibaut Desgeorges, et al.SC79 (Sigma-Aldrich, SML0749), 8 μM TAK-715 (Sigma-Aldrich, SML0360), 200 ng/mL WNT3A (R&D Systems, 5036-WN-010/CF), or 1 μM MK-2206 (Selleckchem, S1078). Immunocytochemistry and image acquisition were performed as

(5) O-GlcNAcylation mediates Wnt-stimulated bone formation by rewiring aerobic glycolysisEMBO reportsOctober 1, 2024You C, Shen F, Yang P, Cui J, Ren Q, Liu M, Hu Y, Li B, Ye L, Shi Y.(FBS) (Zeta) and 1% Pen-Strep (P/S) (Gibco). Recombinant Human Wnt-3a Protein (rhWnt3a) (R&D Systems, 5036-WN-010/CF) was dosed to ST2 cells at 50 ng/ml. Unless otherwise specified, OSMI (Abcam, 20 μM), TMG (APExBIO,

(6) LOXL4, but not LOXL2, is the critical determinant of pathological collagen cross-linking and fibrosis in the lungScience advancesMay 26, 2023Ma HY, Li Q, Wong WR, N'Diaye EN, Caplazi P, Bender H, Huang Z, Arlantico A, Jeet S, Wong A, et al.R&D Systems), TGFβ3 (10 ng/ml; 243-B3-002/CF, R&D Systems), Wnt family member 3a (WNT3A) (500 ng/ml; 5036-WN-010/CF, R&D Systems), lipopolysaccharide (100 ng/ml; 82857-67-8, Sigma-Aldrich), or 20% FBS for 24 hours, followed

(7) WNT5a Signaling through ROR2 Activates the Hippo Pathway to Suppress YAP1 Activity and Tumor GrowthCancer researchApril 4, 2023Wang K, Ma F, Arai S, Wang Y, Varkaris A, Poluben L, Voznesensky O, Xie F, Zhang X, Yuan X, et al.

(8) ASPM Activates Hedgehog and Wnt Signaling to Promote Small Cell Lung Cancer Stemness and ProgressionCancer researchMarch 15, 2023Cheng LH, Hsu CC, Tsai HW, Liao WY, Yang PM, Liao TY, Hsieh HY, Chan TS, Tsai KK.

(9) Novel method of differentiating human induced pluripotent stem cells to mature cardiomyocytes via Sfrp2Scientific reportsMarch 9, 2023Hsueh YC, Pratt RE, Dzau VJ, Hodgkinson CP.

(10) Mucociliary Wnt signaling promotes cilia biogenesis and beatingNature communicationsMarch 6, 2023Seidl C, Da Silva F, Zhang K, Wohlgemuth K, Omran H, Niehrs C.

(11) Age-Dependent Dysregulation of APP in Neuronal and Skin Cells from Fragile X IndividualsCellsFebruary 27, 2023Cencelli G, Pacini L, De Luca A, Messia I, Gentile A, Kang Y, Nobile V, Tabolacci E, Jin P, Farace MG, et al.

(12) CDK5RAP2 is a Wnt target gene and promotes stemness and progression of oral squamous cell carcinomaCell death & diseaseFebruary 11, 2023Shen Y, Chen Y, Lin Y, Li Y, Liu P, Zhang B, Wang Y, Chan KC, Mak NK, Kahn M, et al.

(13) LEF1 Drives a Central Memory Program and Supports Antitumor Activity of Natural Killer T CellsCancer immunology researchFebruary 3, 2023Ngai H, Barragan GA, Tian G, Balzeau JC, Zhang C, Courtney AN, Guo L, Xu X, Wood MS, Drabek JM, et al.

(14) Intravital imaging of Wnt/β-catenin and ATF2-dependent signalling pathways during tumour cell invasion and metastasisJournal of cell scienceFebruary 1, 2023Stoletov K, Sanchez S, Gorroño I, Rabano M, Vivanco MDM, Kypta R, Lewis JD.

(15) Insulin-like growth factor 2 mRNA-binding protein 3 promotes kidney injury by regulating β-catenin signalingJCI insightJanuary 24, 2023Song D, Shang J, Long Y, Zhong M, Li L, Chen J, Xiang Y, Tan H, Zhu H, Hong X, et al.

(16) Nuclear S6K1 Enhances Oncogenic Wnt Signaling by Inducing Wnt/β-Catenin Transcriptional Complex FormationInternational journal of molecular sciencesDecember 18, 2022Lee MG, Oh H, Park JW, You JS, Han JW.

(17) R-spondin 3 deletion induces Erk phosphorylation to enhance Wnt signaling and promote bone formation in the appendicular skeletoneLifeNovember 2, 2022Nagano K, Yamana K, Saito H, Kiviranta R, Pedroni AC, Raval D, Niehrs C, Gori F, Baron R.

(18) Pharmacologic Targeting of TFIIH Suppresses KRAS-Mutant Pancreatic Ductal Adenocarcinoma and Synergizes with TRAILCancer researchSeptember 16, 2022Moser R, Annis J, Nikolova O, Whatcott C, Gurley K, Mendez E, Moran-Jones K, Dorrell C, Sears RC, Kuo C, et al.

(19) WRN promotes bone development and growth by unwinding SHOX-G-quadruplexes via its helicase activity in Werner SyndromeNature communicationsSeptember 16, 2022Tian Y, Wang W, Lautrup S, Zhao H, Li X, Law PWN, Dinh ND, Fang EF, Cheung HH, Chan WY.

(20) Inhibition of phospholipase D1 induces immunogenic cell death and potentiates cancer immunotherapy in colorectal cancerExperimental & molecular medicineSeptember 1, 2022Hwang WC, Song D, Lee H, Oh C, Lim SH, Bae HJ, Kim ND, Han G, Min DS.

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(1)

1/9/2019Low Activity Recombinant Wnt3A Mark EThis was used to induce Wnt signaling in cells amenable to Wnt3a treatment, however activation was far below that seen for L cells secreting Wnt3a.

Recombinant Human Wnt-3a Protein, CF

Wnt-3a Protein

Wnt-3a Protein

Wnt-3a Protein

Wnt-3a Protein

Wnt-3a Protein from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (338)

Reviews

Wnt-3a Protein from R&D Systems, a Bio-Techne Brand

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