Wnt-3a Protein 5036-WN-010 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemWnt-3a Protein
CompanyR&D Systems, a Bio-Techne Brand
Price $452.00Supplier Page View Company Product Page
Catalog Number5036-WN-010
SpeciesHuman
Molecule NameWnt-3a
Size10 ug (also 500 ug)
Source/Expression SystemCHO-derived Recombinant Human Wnt-3a Protein
FormatLyophilized from a 0.2 µm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein
Purity>75%, by SDS-PAGE under reducing conditions and visualized by silver stain
ApplicationsBioactivity
Conjugate/TagUnconjugated
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Sizes Available: 10 ug (also 500 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Generation of Functional Patient-Specific Thymus Organoids From Human Pluripotent Stem Cells (hPSCs) Using Air–Liquid Interface CultureBio ProtocMay 5, 2026Stephan A. Ramos, Holger A. RussVitronectin XFTM (STEMCELL Technologies, catalog number: 07180) 65. Wnt-3a (R&D Systems and Bio-Techne: 5036-WN-010) 66. Antibodies (flow cytometry) a. CD3 (BioLegend, catalog numbers: 317308 and 344818), 1:100 b. CD4

(2) Sustained β-catenin activation via GSK3 inhibition promotes direct fibroblast-to-cardiomyocyte reprogrammingJournal of Biological ChemistryApril 16, 2026Xinghua Wang, Iqra Anwar, Richard E. Pratt, Victor J. Dzau, Conrad P. Hodgkinsonmolecules CHIR99021 (Sigma Aldrich, 5 nM, catalog number SML1046-5 MG), Wnt3a (Novus Biologicals, catalog number 5036-WN-010), Wnt-C59 (Selleck Chemicals, 2 μM, catalog number S7037), TWS-119 (Selleck Chemicals, 2 μM, catalog

(3) Olfactory epithelium organoid models identify Ddit3 as a potential therapeutic target against inflammation-related olfactory sensory neuronal loss and functional deficitInternational Journal of Biological SciencesMarch 30, 2026Jinxia Liu, Jiaming Qi, Nan Jiang, Yuzhen Wang, Weihao Li, Shiyi Tian, Liujing Zhuang, Yunfeng Zhang, Yongliang Liu, Yiqun Yu(200 ng/ml; R&D, #4645-RS), Noggin (100 ng/ml; PeproTech, #250-38), Wnt3a (50 ng/ml; R&D Systems, #5036-WN-010), , 1% N2 (ThermoFisher Scientific, #17502048), 2% B27 (ThermoFisher Scientific, #17504044), and 1%

(4) Wnt3a promotes in situ dentin formation through NKD1-MSX1 axis-mediated odontogenic differentiation of dental pulp stem cellsInternational Journal of Oral ScienceJanuary 13, 2026Haoran Du, Qiong Li, Chenchen Zhou, Junji Xu, Kang Gao, Zixiao Li, Yifan Xu, Ousheng Liu, Bing Li, Jianguang Xu, et al.dexamethasone). Experimental groups received ODM supplemented with 50 ng/mL recombinant human Wnt3a (R&D Systems, 5036-WN-010),10 Wnt5a (Abcam, ab204627), or Wnt10a (Abcam, ab289784). DPSCs were pretreated with 1 μg/mL E. coli

(5) Tegavivint triggers TECR-dependent non-apoptotic cancer cell deathNature Chemical BiologyDecember 1, 2025Logan Leak, Ziwei Wang, Alby J. Joseph, Brianna Johnson, Alyssa A. Chan, Cassandra M. Decosto, Leslie Magtanong, Pin-Joe Ko, Weaverly Colleen Lee, Joan Ritho, et al.chloride, hydroxylamine hydrochloride, sodium fluoride, sodium dodecyl sulfate, purified Wnt3a (Cat# 5036-WN-010, R&D Systems, Minneapolis, MN), LPS, MSB, edelfosine, miltefosine, and perifosine were dissolved in

(6) SAG1.3-derived Frizzled-targeting small-molecule compoundsJournal of Biological ChemistryNovember 1, 2025Lukas Grätz, Ainoleena Turku, Pawel Kozielewicz, Carl-Fredrik Bowin, Magdalena M. Scharf, Jan H. Voss, Julia Kinsolving, Rawan Shekhani, Nuria Oliva-Vilarnau, Tobias Koolmeister, et al.solutions of small-molecule compounds were stored at −20 °C. Lyophilized recombinant WNT-3A (R&D Systems; 5036-WN-010) and high-purity WNT-3A (R&D Systems; 5036-WNP-010) were resuspended in 0.1% bovine serum albumin (Sigma–Aldrich)/Dulbecco’s

(7) A pancreas-hippocampus feedback mechanism regulates circadian changes in depression-related behaviorsNature neuroscienceOctober 1, 2025Liu YN, Wang QW, She XY, Li LJ, Wang B, Yang R, Li Q, Lu SY, Wang YH, Shen W, et al.

(8) Local Growth Hormone Facilitates Aging of the Colon Epithelial MicroenvironmentAging cellOctober 1, 2025Chesnokova V, Zonis S, Ainsworth R, Apaydin T, Valencia CW, Greiner EC, Barrett R, Kettenbach AN, Melmed S.

(9) Establishment of human gastrulating stem cells with the capacity of stable differentiation into multiple gastrulating cell typesCell researchOctober 1, 2025Huang M, Chen M, Yuan G, Cui Y, Shen B, Liu Z, Zhang B, Chen J, Chen D, Qiu S, et al.

(10) Selective and Potent Peptide Binders of RNF43 for Wnt Signaling InhibitionACS Central ScienceSeptember 24, 2025Sunhee Hwang, Paula Flórez Salcedo, Antonion Korcari, John M. Nicoludis, Estefania Martinez Valdivia, Lingling Peng, Aaron T. Balana, Justin Mak, Christopher M. Crittenden, Amin Famili, et al.were seeded onto 12-well plates. The next day, the cells were treated with 50 ng/mL recombinant Wnt3a (5036-WN-010), 25 ng/mL recombinant RSPO2 (3266-RS-025, R&D systems), and monomeric or hexameric DCPs for 6 h. Cells

(11) Cholesterol-targeting Wnt-β-catenin signaling inhibitors for colorectal cancerNature chemical biologySeptember 1, 2025Sharma A, Zalejski J, Bendre SV, Kavrokova S, Hasdemir HS, Ozgulbas DG, Sun J, Pathmasiri KC, Shi R, Aloulou A, et al.

(12) Developing Patient-Derived 3D-Bioprinting models of pancreatic cancerJournal of advanced researchAugust 1, 2025Sun H, Wang Y, Sun M, Ke X, Li C, Jin B, Pang M, Wang Y, Jiang S, Du L, et al.

(13) Differentiation of Human Induced Pluripotent Stem Cells Toward Implantable Chondroprogenitor CellsCARTILAGEJuly 3, 2025Josefine Ekholm, Kristina Vukusic, Camilla Brantsing, Georgina Shaw, Fazal Ur Rehman Bhatti, Stina Simonsson, Anna Falk, Mary Murphy, Victoria Rotter Sopasakis, Anders LindahlSystems,5036-WN,4 µM Day 1,Day 1,Day 1,Day 1 FGF10,R&D Systems,345-FG,10 ng/ml Wnt 3A,R&D Systems,5036-WN-010,10 ng/ml Dorsomorphin dihydrochloride,Bio-Techne,P5499,4 µM Day 2,Day 2,Day 2,Day 2 FGF10,R&D Systems,345-FG,10

(14) Redefining the role of IL-18 in post-surgical recovery and sepsis: a key mediator of inflammation resolutionJournal of translational medicineJuly 2, 2025Papatheodorou I, Blažková G, Bosáková V, Tomášiková Z, Spearing E, Klieber R, Ostašov P, Štíchová J, Dvončová M, Mýtniková A, et al.

(15) WNT-induced association of Frizzled and LRP6 is not sufficient for the initiation of WNT/β-catenin signalingNature communicationsMay 24, 2025Voss JH, Koszegi Z, Yan Y, Shorter E, Grätz L, Lanner JT, Calebiro D, Schulte G.WNTs and R-spondin 1 were purchased from Biotechne with the following catalog numbers: human WNT-3A (5036-WN-010), human/mouse WNT-5A (645-WN-010/CF), recombinant human WNT-10B (7196-WN-010), human WNT-16B (7790-WN-025),

(16) Deficiency of Aging‐Related Gene Chitinase‐Like 4 Impairs Olfactory Epithelium HomeostasisCell ProliferationMay 19, 2025Tingting Wu, Weihao Li, Liujing Zhuang, Jinxia Liu, Ping Wang, Ye Gu, Yongliang Liu, Yiqun Yu(200 ng/mL; R&D, #4645‐RS), Noggin (100 ng/mL; PeproTech, #250–38), Wnt3a (50 ng/mL; R&D Systems, #5036‐WN‐010), Y27632 (10 μM; Sigma‐Aldrich, #Y‐0503), human epidermal growth factor (50 ng/mL; ThermoFisher Scientific,

(17) Enhanced viability and functional maturity of iPSC-derived islet organoids by collagen-VI-enriched ECM scaffoldsCell stem cellApril 3, 2025Zhu D, Chen Z, Guo K, Xie Q, Zou Y, Mou Q, Zhou Z, Jin G.

(18) Robust and reproducible human intestinal organoid-derived monolayer model for analyzing drug absorptionScientific reportsApril 3, 2025Tanaka K, Mochizuki T, Baba S, Kawai S, Nakano K, Tachibana T, Uchimura K, Kato A, Miyayama T, Yamaguchi T, et al.10 mM nicotinamide (Sigma), 10% Afamin/Wnt3a CM (J2-001; JSR)59 or 50 ng/mL recombinant human Wnt3a (5036-WN-010; R&D systems), 1 μg/mL recombinant human R-spondin-1 (120–38; PeproTech), 100 ng/mL animal-free recombinant human EGF

(19) SOX9 haploinsufficiency reveals SOX9-Noggin interaction in BMP-SMAD signaling pathway in chondrogenesisCell Mol Life SciMarch 2, 2025Tin-Yan Ha, See-Wing Chan, Zhangting Wang, Patrick Wai Nok Law, Kai-Kei Miu, Gang Lu, Wai-Yee Chan2-mercaptolethanol (Gibco™, 21985023). 7 chemicals, Recombinant Human Wnt-3a Protein (R&D Systems, 5036-WN-010), Recombinant Human/Murine/Rat Activin A (PEPROTECH, 120-14P), bFGF (Thermo Scientific™, PHG6015), BMP4

(20) Wnt signaling inhibits casein kinase 1α activity by modulating its interaction with protein phosphatase 2ACell ReportsFebruary 25, 2025Chen Shen, Wenhui Lu, Siva B. Merugu, Aradhana Bharti, Said M. Afify, Lauren Schnitkey, Daniel T. Wynn, Fan Yang, Thomas M. Rohwetter, Anmada Nayak, et al.recombinant proteins","Chemicals, peptides and recombinant proteins" Recombinant Wnt3a,R&D Systems,Cat# 5036-WN-010 Recombinant Wnt3a,Time Bioscience,Cat# rhW3aH Recombinant Frizzled8-CRD,R&D Systems,Cat# 6129-FZ Recombinant

Write a Review

(1)

9/20/2021Good for Measuring Wnt3a Luciferase Activity Daan VieringThis product worked very well when we treat it to 29SFT cells.
(1)

1/9/2019Low Activity Recombinant Wnt3A Mark EThis was used to induce Wnt signaling in cells amenable to Wnt3a treatment, however activation was far below that seen for L cells secreting Wnt3a.

Recombinant Human Wnt-3a Protein

Wnt-3a Protein

Wnt-3a Protein

Wnt-3a Protein

Wnt-3a Protein

Wnt-3a Protein from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (447)

Reviews

Wnt-3a Protein from R&D Systems, a Bio-Techne Brand

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