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Bacterial Viability / Cytotoxicity Stains
7-AAD Viability Staining Solution
7-AAD Viability Staining Solution from BioLegend
(2)
Write a review
Citations:
(438)
Supplier Page
Supplier Page from
BioLegend for
7-AAD Viability Staining Solution
Description
7-AAD Viability Staining Solution
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
7-AAD Viability Staining Solution
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
420403
Applications
Flow Cytometry (FC)
Quantity
200 tests
Format
PBS, pH 7.2 with 0.09% (w/v) sodium azide
Target
7-AAD
Concentration
50.00 µg/mL
Add to Compare List
BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (438)
(1)
LFA-1 regulated by IL-2/STAT5 pathway boosts antitumor function of intratumoral CD8+ T cells for improving anti-PD-1 antibody therapy
Oncoimmunology
December 21, 2023
Jiqi Shan, Wei Jing, Yu Ping, Chunyi Shen, Dong Han, Fengsen Liu, Yaqing Liu, Congcong Li, Yi Zhang
stained with Annexin V (
BioLegend
, cat. #
640920
) and
7-AAD Viability Staining Solution
(
BioLegend
, cat. #
420404
).
Flow cytometry
was used to analyze the lysis of B16
tumor
cells.
Elisa
The IFN-γ and IL-2 production
(2)
Bruton's tyrosine kinase-bearing B cells and microglia in neuromyelitis optica spectrum disorder
J Neuroinflammation
December 21, 2023
Ye Liu, Zhenning Huang, Tian-Xiang Zhang, Bin Han, Guili Yang, Dongmei Jia, Li Yang, Qiang Liu, Alexander Y. L. Lau, Friedemann Paul,
et al.
solution (
BioLegend
,
422302
) and thereafter, incubated with
7-AAD Viability Staining Solution
(
BioLegend
,
420403
), CD3 (BioLegend,
clone
OKT3), CD14 (BioLegend,
clone
63D3) and CD16 (BioLegend,
clone
3G8). B cells
(3)
CEA cell adhesion molecule 5 enriches functional human hematopoietic stem cells capable of long-term multi-lineage engraftment
iScience
December 15, 2023
Kuiying Ma, Xuan Wang, Linjie Wu, Lingling Yu, Jinhui Ye, Xueling Li, Lili Geng, Zhongyu Shi, Huihui Yang, Xijuan Zhang,
et al.
buffer,
Biolegend
,Cat:
420301
TRIzol reagent
,
Invitrogen
,Cat:
15596018
7-AAD Viability Staining Solution
,
Biolegend
,Cat:
420404
,, Critical commercial
assays
,Critical commercial
assays
,Critical commercial
assays
,,
CD34
+ microbead
(4)
BR109, a Novel Fully Humanized T-Cell-Engaging Bispecific Antibody with GPRC5D Binding, Has Potent Antitumor Activities in Multiple Myeloma
Cancers (Basel)
December 9, 2023
Ying Liu, Ya-Qiong Zhou, Lei Nie, Shan-Shan Zhu, Na Li, Zhen-Hua Wu, Qi Wang, Jian Qi, Bing-Yuan Wu, Shu-Qing Chen,
et al.
the cells were washed, 5 µL of
7-AAD Viability Staining Solution
(
Biolegend
, San Diego, CA, USA, CAT#
420404
) was added to obtain a final
staining
volume of 200 μL. The cells were gently vortexed and incubated
(5)
The Defined TLR3 Agonist, Nexavant, Exhibits Anti-Cancer Efficacy and Potentiates Anti-PD-1 Antibody Therapy by Enhancing Immune Cell Infiltration
Cancers (Basel)
December 8, 2023
Seung-Hwan Lee, Young-Ho Choi, Soon Myung Kang, Min-Gyu Lee, Arnaud Debin, Eric Perouzel, Seung-Beom Hong, Dong-Ho Kim
PerCP/Cy5.5-conjugated anti-
mouse
Ly6G
mAb (Cat. #:
127615
), and
7-AAD Viability Staining Solution
(Cat. #:
420404
) were purchased from
BioLegend
(San Diego, CA, USA). 2.4. Treatment with Nexavant and Poly(I:C) When
(6)
Immune checkpoints are predominantly co-expressed by clonally expanded CD4+FoxP3+ intratumoral T-cells in primary human cancers
J Exp Clin Cancer Res
December 6, 2023
Delphine Bredel, Edi Tihic, Séverine Mouraud, François-Xavier Danlos, Sandrine Susini, Marine Aglave, Alexia Alfaro, Chifaou Mohamed-Djalim, Mathieu Rouanne, Héloise Halse,
et al.
anti-CD45-FITC mAbs (
Biolegend
,
304038
), washed, and resuspended in PBS 0,04% BSA. 7-AAD (
Biolegend
,
420404
) was added to the cells prior to sorting. At least 50,000 live
CD45
positive cells were sorted in PBS
(7)
Protocol for high-sensitivity western blot on murine hematopoietic stem cells
STAR Protocols
December 1, 2023
Changzheng Li, Jingjing Guan, Yishan Li, Xiaobin Tian, Yijun Zhao, Weiming Liu, Huixuan Tian, Huiqi Tian, Yalan Yang, Meng Zhao
Biotech,Cat#A501195-0500
7-AAD viability staining solution
(working concentration: 0.5 μg per mL),
BioLegend
,Cat#
420404
BSA,
Sigma
,Cat#A1933-5G 30% Acr/Bis (29:1) glue solution,Meilunbio,Cat#MA0071
Tris
,"Beijing Dingguo
(8)
Detection of the CD8+ T cell immune response in mice infected with OVA-Listeria monocytogenes
STAR Protocols
December 1, 2023
Yudai Xu, Zijian Wang, Wen Gao, Haoyun Wang, Zhixian Wang, Chenxuan Tian, Guobing Chen
Tetramer-SIINFEKL-PE,MBL Life science,Code No. TS-5001-1C
7-AAD Viability Staining Solution
,
BioLegend
,Cat#
420404
Zombie NIR Fixable Viability Kit,
BioLegend
,Cat#
423105
Brain heart infusion (BHI),Oxoid,Cat# CM1135 Agar,Biosharp,Cat#
(9)
Unbiased assessment of genome integrity and purging of adverse outcomes at the target locus upon editing of CD4+ T‐cells for the treatment of Hyper IgM1
The EMBO Journal
December 1, 2023
Daniele Canarutto, Claudia Asperti, Valentina Vavassori, Simona Porcellini, Elisabetta Rovelli, Marianna Paulis, Samuele Ferrari, Angelica Varesi, Martina Fiumara, Aurelien Jacob,
et al.
Pharmingen
,C40‐1457,
560326
,
1:25
CD154
,PE,Invitrogen,24‐31,12‐548‐42,5:100
CD154
,PE,
Biolegend
,24‐31,
310806
,
1:50
7‐AAD Viability Staining Solution
,7‐AAD,
Biolegend
,‐,
420404
,
1:100
(10)
Single-cell analysis reveals key differences between early-stage and late-stage systemic sclerosis skin across autoantibody subgroups
Annals of the Rheumatic Diseases
December 1, 2023
Kristina Elizabeth Neergaard Clark, Shiwen Xu, Moustafa Attah, Voon H Ong, Christopher Dominic Buckley, Christopher P Denton
liquid nitrogen after 24 hours. Thawing Thawing occurred just prior to 7-AAD
staining
(
BioLegend
420404
) and viable
cell sorting
by the SH800 Cell sorter (Sony Biotechnology). Viable cells in single-cell
(11)
Monoclonal enolase-1 blocking antibody ameliorates pulmonary inflammation and fibrosis
Respiratory Research
November 14, 2023
Wei-Ching Huang, Chi-Fen Chuang, Yung-Tsang Huang, I-Che Chung, Mao-Lin Chen, Tung-Yueh Chuang, Xiu-Li Yang, Yu-Yau Chou, Chih-Hsin Liu, Nai-Yu Chen,
et al.
APC-streptavidin
(#
405207
,
BioLegend
) at 4 oC for 30 min, followed by washing and
staining
with 7-AAD (#
420404
,
BioLegend
). Samples were analyzed on a
FACSCanto
II
flow cytometer
(
BD Biosciences
, San Jose, CA, USA)
(12)
Humanized single-domain antibody targeting HER2 enhances function of chimeric antigen receptor T cells
Frontiers in Immunology
November 7, 2023
Rui Zheng, Yuankun Chen, Yiting Zhang, Sixin Liang, Xiaojuan Zhao, Yiyi Wang, Pengju Wang, Ruotong Meng, Angang Yang, Bo Yan
V (
1:20
, Cat#
640951
,
BioLegend
) and its binding buffer (Cat#
422201
,
BioLegend
), 7-AAD (
1:20
, Cat#
420404
,
BioLegend
), Granzyme B (
1:20
, Cat#
372206
,
BioLegend
) were used. A total of 1 × 106 cells per group
(13)
Loss of Cadherin-11 in pancreatic ductal adenocarcinoma alters tumor-immune microenvironment
Frontiers in Oncology
October 26, 2023
Aimy Sebastian, Kelly A. Martin, Ivana Peran, Nicholas R. Hum, Nicole F. Leon, Beheshta Amiri, Stephen P. Wilson, Matthew A. Coleman, Elizabeth K. Wheeler, Stephen W. Byers,
et al.
minutes in the dark. Cells were resuspended in
7-AAD Viability Staining Solution
(
BioLegend
, Catalog #
420404
) and analyzed on a BD FACSMelody cytometer, sorting
CD45
+/7-AAD- and
CD45
+/7-AAD- populations for
scRNA-seq
(14)
Single-cell profiling coupled with lineage analysis reveals vagal and sacral neural crest contributions to the developing enteric nervous system
eLife
October 25, 2023
Jessica Jacobs-Li, Weiyi Tang, Can Li, Marianne E Bronner
The cells were resuspended in HBSS-BSA, supplemented
7-AAD Viability Staining Solution
(
BioLegend
#
420404
, 500 TESTS), and sorted for YFP+, viable single cells using Sony SY3200 cell sorter at the Caltech
Flow Cytometry
(15)
Increased epithelial mTORC1 activity in chronic rhinosinusitis with nasal polyps
bioRxiv
October 21, 2023
George X. Huang, Nils R. Hallen, Minkyu Lee, Kelly Zheng, Xin Wang, Michael V. Mandanas, Sarah Djeddi, Daniela Fernandez, Jonathan Hacker, Tessa Ryan,
et al.
(BioLegend 2434),
CD45
(
BioLegend
103116
), and
CD90
(
BioLegend
105328
) for 30 min. 7AAD (
BioLegend
420403
) was added immediately before
cell sorting
. After removing debris, doublets, and dead cells,
EpCAM
+
(16)
Venetoclax Overcomes Sorafenib Resistance in Acute Myeloid Leukemia by Targeting BCL2
Biology (Basel)
October 16, 2023
Xi Xu, Weiwei Ma, Guo Qiu, Li Xuan, Chong He, Tian Zhang, Jian Wang, Qifa Liu
growth curves were plotted using
flow cytometry
to count viable cells, and 7-aminoactinomycin D (7-AAD,
420404
,
Biolegend
, San Diego, CA, USA) was used to exclude dead cells. For apoptosis analysis, 1 × 106 cells
(17)
TIM-3 Expression and M2 Polarization of Macrophages in the TGFβ-Activated Tumor Microenvironment in Colorectal Cancer
Cancers (Basel)
October 11, 2023
Masanori Katagata, Hirokazu Okayama, Shotaro Nakajima, Katsuharu Saito, Takahiro Sato, Mei Sakuma, Satoshi Fukai, Eisei Endo, Wataru Sakamoto, Motonobu Saito,
et al.
Pacific Blue-conjugated anti-human CD86 mAb (
305423
,
BioLegend
),
7-AAD Viability Staining Solution
(
420404
,
BioLegend
), PE-conjugated anti-human TIM-3 mAb (
345005
,
BioLegend
) and PE-conjugated isotype control
(18)
Short-term assays for mesenchymal stromal cell immunosuppression of T-lymphocytes
Frontiers in Immunology
September 26, 2023
Maryanne C. Herzig, Barbara A. Christy, Robbie K. Montgomery, Carolina Cantu-Garza, Gema D. Barrera, Ji H. Lee, Nicholas Mucha, Jennifer R. Talackine, Isaac A. Abaasah, James A. Bynum,
et al.
the
lymphocyte
population, uptake of the nucleic acid dye 7-aminoactinomycin D (7AAD;
BioLegend
Cat #
420404
) discriminated live/dead
staining
. Specifically, cells were centrifuged at 400 × g for 6 min, resuspended
(19)
Unravelling human hematopoietic progenitor cell diversity through association with intrinsic regulatory factors
bioRxiv
September 7, 2023
Patricia Favaro, David R. Glass, Luciene Borges, Reema Baskar, Warren Reynolds, Daniel Ho, Trevor Bruce, Dmitry Tebaykin, Vanessa M. Scanlon, Ilya Shestopalov,
et al.
EQ, Four Element",Standard BioTools Inc.,Cat#201078
7-AAD Viability Staining Solution
,
BioLegend
,Cat#
420404
,, Deposited Data,Deposited Data,Deposited Data ,,
Mass cytometry
batch-corrected fcs files,This
(20)
Discovery and multimerization of cross-reactive single-domain antibodies against SARS-like viruses to enhance potency and address emerging SARS-CoV-2 variants
Sci Rep
August 22, 2023
Scott A. Hollingsworth, Cameron L. Noland, Karin Vroom, Anasuya Saha, Miranda Sam, Qinshan Gao, Haihong Zhou, David U. Grandy, Sujata Singh, Zhiyun Wen,
et al.
goat anti-llama IgG (H + L) FITC
conjugate (
Thermo
Cat#
A16061
), 7-AAD live/dead
cell stain
(
Biolegend
, Cat#
420404
), and streptavidin BV421 (
Biolegend
Cat#
405226
). Live B-cells, positive for biotinylated antigen and
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Reviews
Write a Review
(1)
3/13/2019
Staining Human T Cells with 7-AAD
Adam Guess
This reagent was used along with anti-Ki67 for cell cycle analysis in stimulated human T cells. It was chosen because it is a commonly used DNA stain, 7-AAD works well with other fluorophores in the ...
Read Review
(1)
10/29/2015
Cell Viability Stain
Sachin Samuchiwal
I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from the mouse system using anti-mycobacterial ESAT6 binding peptides.
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7-AAD Viability Staining Solution from BioLegend
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View Company Product Page
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