A wide variety of enzymes are available for research applications in cellular and molecular biology. To narrow down your search, select desired filters or enter keywords in the provided text boxes. It may be helpful to note the enzyme source, reaction conditions and units of activity provided by the manufacturers before making a purchase.
The T4 DNA Polymerase is a DNA polymerase originally isolated from the T4 Bacteriophage. Like the E. coli DNA Polymerase I, it has a 5’-3’ polymerase activity active at 37 °C. It features a particularly stronger 3’-5’ exonuclease proofreading activity that is more active on single stranded DNA than on double stranded. In addition, the polymerase activity requires a primed single strand of DNA, duplex DNA with gaps, or single stranded DNA with a protruding 5’ end. This polymerase is recommended for blunting, site-specific mutagenesis, and DNA gap filling.