T4 DNA Polymerase from Promega

Product Specs
ItemT4 DNA Polymerase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberM4215
Quantity500u
Concentration5–10u/µl
Molecular Weight~66kDa

Specifications/Features

Unit Definition:
One unit is defined as the amount of enzyme required to catalyze the incorporation of 55nmol of dTTP into acid-precipitable material in 30 minutes at 39°C using poly(dA):oligo(dT) as a substrate.

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) A remarkably diverse and well-organized virus community in a filter-feeding oysterMicrobiomeJanuary 7, 2023Jing-Zhe Jiang, Yi-Fei Fang, Hong-Ying Wei, Peng Zhu, Min Liu, Wen-Guang Yuan, Li-Ling Yang, Ying-Xiang Guo, Tao Jin, Mang Shi, et al.fragments of ≤ 800 bp. The sticky ends were repaired, and adapters were added using T4 DNA polymerase (M4211, Promega, USA), Klenow DNA polymerase (KP810250, Epicentre), and T4 polynucleotide kinase (EK0031, Thermo Fisher Scientific

(2) Metagenome-Assembled Viral Genomes Analysis Reveals Diversity and Infectivity of the RNA Virome of Gerbillinae SpeciesVirusesFebruary 9, 2022Han Du, Lijuan Zhang, Xinqiang Zhang, Fengze Yun, Yuhao Chang, Awaguli Tuersun, Kamila Aisaiti, Zhenghai Maproduce fragments of ≤800 bp; and sticky ends were repaired, and adapters were added using T4 polymerase (M4211, Promega, Madison, WI, USA), Klenow DNA Polymerase (KP810250, Epicentre), and T4 polynucleotide kinase

(3) The Husavirus Posa-Like Viruses in China, and a New Group of PicornaviralesVirusesSeptember 7, 2020Zhenzhi Han et al.produce 800 bp fragments, then sticky ends were repaired and adapters were added using T4 DNA polymerase (M4211, Promega, Madison, WI, USA), Klenow DNA Polymerase (KP810250, Epicentre, Woburn, MA, USA), and T4 polynucleotide kinase

(4) Viral Metagenomics Revealed Sendai Virus and Coronavirus Infection of Malayan Pangolins (Manis javanica)VirusesOctober 24, 2019Ping Liu, Wu Chen, Jin-Ping Chenproduce fragments of ≤ 800 bp, and sticky ends repaired and adapters added using T4 DNA polymerase (M4211, Promega, Madison, WI, USA), Klenow DNA Polymerase (KP810250, Epicentre), and T4 polynucleotide kinase

(5) Long-read ChIA-PET for base-pair-resolution mapping of haplotype-specific chromatin interactionsNature ProtocolsMay 1, 2017Xingwang Li et al.no. 04707516001) T4 DNA ligase (Thermal, cat. no. EL0013) T4 DNA Polymerase (Promega, cat. no. M4215) Tris-HCl, pH 7.0 (1 M, 500ml, Ambion, cat. no. AM9856) Triton-X-100, Molecular Biology Grade (Promega,

(6) Immunogenic Properties of Streptococcus agalactiae FbsA FragmentsPLoS ONESeptember 24, 2013Salvatore Papasergi et al.average size of approximately 300 bps were then manually cut from gels, filled in with T4 DNA polymerase (M4211; Promega, Milan, Italy) and ligated with specific adaptors into vector λKM4, to be cloned as fusion

(7) Chromatin Interaction Analysis Using Paired-End Tag SequencingCurrent Protocols in Molecular BiologyJanuary 1, 2010Melissa J. , Yuyuan , Chia-Lin , Xiaoan , Yijun

(8) RNA-Seq: A Method for Comprehensive Transcriptome AnalysisCurrent Protocols in Molecular BiologyJanuary 1, 2010Ugrappa , Karl , Michael

(9) Phloem Unloading of Potato virus X Movement Proteins Is Regulated by Virus and Host FactorsMolecular Plant-Microbe InteractionsÂ®August 1, 2008Tefera Mekuria, Devinka Bamunusinghe, Mark Payton, Jeanmarie Verchot-Lubicz

(10) Paired-end diTagging for transcriptome and genome analysisCurrent protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]July 1, 2007Patrick Ng, Chia-Lin Wei, Yijun Ruan

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RNA- Seq: A Method for Comprehensive Transcriptome Analysis
January 01, 2010
Chromatin Interaction Analysis Using Paired- End Tag Sequencing
January 01, 2010
Paired- End diTagging for Transcriptome and Genome Analysis
July 01, 2007