Superb SYBR Green Master Mix From Thermo Fisher Scientific

February 07, 2024

University of South Florida
College of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

PowerTrack™ SYBR Green Master Mix for qPCR

Catalog Number:

A46012

We study the CNS basement membrane and blood-brain barrier. Specifically the role of different CNS cell types in BBB maintenance.

Experimental Design and Results Summary

Application

Gene Expression, Virus Detection

Starting Material

RNA

Tips

Used for 100 Reactions

Results Summary

PowerTrack SYBR Green Master Mix is a pre-formulated, optimized, universal 2X master mix for real-time PCR workflows with built-in visual dye–based indicators for accurate reaction set up. Coupled with user-supplied primer sets and templates, PowerTrack SYBR Green Master Mix is designed to amplify targets for accurate gene expression analysis. We routinely use the SYBR Green Master Mix Gene Expression studies. This mix provides robust and efficient results in qPCR studies.

DOI or PMID #

N/A

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Summary

The Good

Built-in visual indicators aid in reaction setup, Broad primer Tm and concentration compatibility allows qPCR reaction setup flexibility with minimal optimization, Specific and tight reproducibility in Ct’s over a broad dynamic range, Formulated with UNG/dUTP to prevent contamination of carryover PCR products.

The Bad

Higher Cost.

The Bottom Line

Easy setup and results.

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