Fast and Versatile Reagent From ThermoFisher for Embryoid Body Dissociation

December 28, 2023

University of Toronto
Chemical/Biomedical Engineering
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

ThermoFisher Scientific/Gibco

Product Name:

TrypLE™ Express Enzyme (1X), no phenol red

Catalog Number:

12604-013

Embryoid bodies (EBs) were formed from human embryonic stem cells for mesoderm differentiation. TryplE was used to dissociate EBs after mesoderm differentiation for monolayer culture and further differentiation steps.

Experimental Design and Results Summary

Application

Dissociation of embryoid bodies into single cell suspension

Starting Material

HES2 EBs

Tips

May need to mechanically agitate cells a few times during incubation, incubate at 37 degrees

Results Summary

TryplE facilitated successful dissociation of mesoderm differentiation EBs that were able to be plated as a monolayer and differentiated into epicardial cells, displaying characteristic markers.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Simple to use - add directly to cells with no other factors needed. Faster dissociation times than other reagents.

The Bad

May damage cells if incubated for too long. Still required mechanical agitation throughout incubation for proper dissociation.

The Bottom Line

A simple and easy-to-use dissociation reagent for EB culture and stem cell differentiation. Would recommend for reproducible and reliable results.

Join the discussion