Anti-Tumor necrosis factor antibodies are used for the immunodetection of the protein encoded by the TNF gene. In humans, the canonical protein has a reported length of 233 amino acid residues and a mass of 25.6 kDa. Its subcellular localization is in the cell membrane and is secreted. A member of the Tumor necrosis factor protein family, it is a reported to be a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. Post-translational modifications have been described, including O-glycosylation, protein cleavage, myristoylation and phosphorylation.
The tumor necrosis factor marker can be used to characterize CD4 Memory T Cells and Effector Memory CD45RA CD4 T Cells.* Other names for this target antigen include TNF-alpha, TNFA, TNFSF2, TNLG1F, APC1 protein, TNF, macrophage-derived, TNF, monocyte-derived, and DIF. Gene orthologs have been identified in the mouse, rat, bovine, frog and chimpanzee species. Over 6200 citations in the literature describe the use of Tumor necrosis factor antibodies in research. ELISA is a widely used application for these antibodies. In addition, Western Blot is also a common application.
*HuBMAP Human Reference Atlas v1.4