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APC anti-mouse TNF-α Antibody
506308
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This antibody was used for intracellular staining of TNF-alpha on mouse CD8 T cells that were stimulated with OVA peptide. This seems to be a strong stimulation as most cells were positive for TNF-alpha after 3 days of stimulation. Usually, with a polyclonal stimulus, only a fraction of cells may express TNF-alpha. I recommend using different stimulation conditions to standardize the staining of this antibody.
Flow Cytometry
Purified mouse CD8 T cells
1:200 dilution for 1 hour on ice
NA
Flow cytometry on APC channel
The results show uniformly high expression of TNF-alpha on all cells, which is unexpected. There may be some background staining, needs optimizing.
None
Expression levels are high
Need to optimize under different stimulation conditions
The antibody can detect TNF-alpha. The staining and stimulation methods need optimization.