Anti-mouse TNF-alpha PE-Cy7 for Intracellular Staining

Cancer Biology
Scripps research center in FL
Research Associate

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Company:

BioLegend

Product Name:

TNF-apha PE-Cy7

Catalog Number:

506324

We transfer wild type CD4 T cells to Rag-/- mice to establish Colitis model and then look at the phenotype of these transferred CD4 T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

1:200 dilution

Blocking Agent

PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS LSRII

Results Summary

After fixation and permeabilization, spleen cells were stained by this Ab. TNF-alpha production was evaluated in CD4+CD25- T cells. Most of the cells expressed TNF-alpha

Additional Notes

None

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Summary

The Good

Easy application

The Bad

None

The Bottom Line

Can be used to detect TNF-alpha production by FACS.

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