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Date Published
Handy Filter Sterilization of Culture Media From Millipore Sigma
(1)
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MilliporeSigma
July 26, 2023
Cell culture media prepared with non-sterile reagents can be filtered through the Steriflip unit for filter sterilization before application...
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Simple Centrifugal Filter Unit for Ultrafiltration of Culture Media From Millipore Sigma
(1)
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MilliporeSigma
July 26, 2023
Cells were cultured in monolayers for extracellular vesicle (EV) isolation. Centrifugal filter units (100kDa size cutoff) were used to ...
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Substrate for Alkaline Phosphatase Assay From Sigma Aldrich
(1)
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MilliporeSigma
July 25, 2023
Hedgehog signaling plays a critical role in embryonic development. It also controls adult tissue homeostasis and deregulation of this ...
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Di-tert-butyl dicarbonate for Boc Protection
(1)
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MilliporeSigma
July 23, 2023
The aim was to protect just one side of a diamine to be subsequently able to link it to a second scaffold.
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Synthesis of Schiff Base
(1)
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MilliporeSigma
July 23, 2023
We synthesized a Schiff base using an amine and an aldehyde. This product will be then linked to a larger scaffold exploiting the imine ...
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Effective Reducing Agent From Sigma Aldrich
(1)
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MilliporeSigma
July 23, 2023
This product was used to reduce ester to aldehydes avoiding the risk to get the corresponding alcohol
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Great Antibody for Immunofluorescence From Millipore Sigma
(1)
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MilliporeSigma
July 22, 2023
Blood vessels in the brain of mice are identified by staining for basement membrane glycoprotein laminin. Regions, where the basement ...
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Purification of His-Tagged Proteins
(1)
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MilliporeSigma
July 20, 2023
I am interested in studying the molecular mechanism of oncogenic WNT signal transduction to identify potential targets for therapeutic ...
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APC Antibody Works for Western Blotting
(1)
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MilliporeSigma
July 20, 2023
The WNT pathway is essential for embryonic development and adult tissue homeostasis. Deregulation of this pathway results in cancers of the ...
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Great FLAG Antibody From Sigma Aldrich
(1)
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MilliporeSigma
July 17, 2023
It is a great antibody used to detect expression levels of a protein that was FLAG-tagged for purification for cryoEM studies.
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Good Anti-HA tag Antibody (H3663)
(1)
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MilliporeSigma
July 11, 2023
To identify an HA-tag primary antibody to detect the exogenous expression of the constructed plasmids in OE19 stable cell models, five HA-...
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Working Great in Fibroblasts
(1)
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MilliporeSigma
July 02, 2023
I used a Chloroquine diphosphate salt from Sigma-Aldrich to block autophagosome degradation. I found this one which we purchased in 2010, it...
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Glacial Acetic Acid 100%, Water Free
(1)
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MilliporeSigma
June 30, 2023
We bought the Glacial acetic acid because it is 100% water-free acid, so it is more concentrated. I used the reagent within the ...
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Acid Catalyzed Reaction with Titanium (III) Chloride
(1)
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MilliporeSigma
June 01, 2023
We tried to get a intramolecular cyclization using TiCl3 as catalyst. Its purpose was to activate the carboxylic functional moiety of the ...
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Specific Staining with Neurofilament 200 Antibody
(1)
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MilliporeSigma
May 23, 2023
I'm using it on immunohistochemical staining. it is working on 1/250 dilution rate in rat, dog and sheep tissues. There are some background ...
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Bulky Base Works Well for Deprotonation
(1)
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MilliporeSigma
May 12, 2023
Synthesis of new peptide bond starting from an acyl chloride and an amide in a dry atmosphere of N2, using bulky base.
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Deprotonation of Mild Acid Protons with Butyllithium
(1)
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MilliporeSigma
May 09, 2023
The product has been used to deprotonate protons with high pKa that are not possible to deprotonate with more mild bases.
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Synthesizing New Peptide Bond with Acryloyl Chloride
(1)
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MilliporeSigma
May 04, 2023
Synthesis of new peptide bond starting from acyl chloride and an amine. I used 1 equivalent added at 0 °C and then brought to room ...
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Good P-SMAD2 Antibody for Immunostaining
(1)
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MilliporeSigma
May 03, 2023
Smad proteins SMAD2 and SMAD3 are core transcription factors involved in TGF-beta signaling, and they interact with cofactors to control the...
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Great ZNF518B Antibody for Western Blotting
(1)
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MilliporeSigma
May 03, 2023
Good antibody for detecting ZNF518b in MCF10a cells. Little background noise and can reuse multiple times.
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Good Actin Antibody for Western Blot Control
(1)
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MilliporeSigma
April 27, 2023
I used this as a loading control for western blots. It worked very well since I was looking at proteins of higher and lower molecular ...
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Chloroform-d Works Well for NMR Spectra
(1)
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MilliporeSigma
April 25, 2023
After the end of any reaction, there are several methods that can be used to check if it is gone to the right way or not. One of this ...
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Do Not Trust the Literature on This Ubiquitinylated Protein
(1)
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MilliporeSigma
April 19, 2023
I tried 4 different Ub antibodies (two of which have been published on in my field and protein of interest, including this one) and this one...
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Loading Control Works Every Time
(1)
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MilliporeSigma
April 12, 2023
This mouse mAb against beta-Actin is our daily-use loading control for Western Blot detection. The concentration needed is very low, the ...
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Great GFAP Antibody for IF
(1)
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MilliporeSigma
March 20, 2023
Astrocyte activation in the olfactory bulb of mice infected with respiratory virus is analyzed by GFAP staining of brain sections.
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Lysis of Red Blood Cells in the Isolation of PBMCs
(1)
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MilliporeSigma
March 20, 2023
Red Blood Cell Lysis Buffer has been used in our laboratory for the lysis of red blood cells in the isolation of PBMCs (peripheral blood ...
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Great Anti-Olig2 Antibody for Immunofluorescence
(1)
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MilliporeSigma
March 01, 2023
This antibody was used to label the oligodendrocyte percursor cells in a developing mouse brain tissue.
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Great Anti-acetyl-Histone H3 Antibody for Western Blot
(1)
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MilliporeSigma
February 24, 2023
Very good antibody - clean bands with little background. Easy to use. One of the best I have used for acetylated-H3.
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Reliable Collagenase XI for Tissue Digestion
(1)
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MilliporeSigma
February 22, 2023
The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the ...
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Highly Recommended Dispase II for Tissue Digestion
(1)
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MilliporeSigma
February 22, 2023
The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the ...
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Anti-a-SMA Antibody (A5228) Works Well
(1)
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MilliporeSigma
February 22, 2023
Foxp1(Forkhead box transcription factor P1)ECKO mutants exhibit greater AngII-induced cardiac fibrosis by elevated expression of a-SMA and ...
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Good NMT1 Antibody from MilliporeSigma
(1)
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MilliporeSigma
February 15, 2023
NMT1 was knockdown in PC-3 cell lines by shRNA. Antibody was used to detect the knockdown efficiency. The results showed that this antibody...
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Useful Chromatin Immunoprecipitation (ChIP) Assay Kit
(1)
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MilliporeSigma
February 13, 2023
Upstate Biotechnology’s Chromatin Immunoprecipitation (ChIP) Assay Kit now has the cat # 17-295 since it was merged by Sigma-Aldrich. ...
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Excellent Alternative Cell Freezing Solution
(1)
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MilliporeSigma
February 03, 2023
Anyone who performs cell culture knows the importance of having a reliable way to freeze down cells. the standard solution of 10% DMSO in ...
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Great to Identify Exogenous Tf
(1)
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MilliporeSigma
January 30, 2023
I used this to identify exogenous Tf in a solution. Importantly, this is holo-Tf (iron bound). Since I study differences between apo- and ...
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RIPA is an Efficient and Ready to Use Cell Lysis Buffer
(1)
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MilliporeSigma
January 30, 2023
We use RIPA Buffer as a reagent for cell lysis when preparing cells lysate for protein quantification. This product is very useful as is ...
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Amazing Technique to Show Protein Interactions
(1)
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MilliporeSigma
January 23, 2023
I love this kit and technique to show protein-protein interactions. It formed the basis of my most recent publication. The kit is very easy ...
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Reasonably Good Antibody to Detect GFP-Tag
(1)
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MilliporeSigma
January 19, 2023
We used this antibody to detect GFP tags in western blot applications. The antibody is not highly specific and works well at the suggested ...
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Efficient Mitomycine C for Cell Culture
(1)
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MilliporeSigma
December 29, 2022
I use this product for research about immune response between PBMCs and target cells. When I have to use immortal cell lines, I must use ...
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Great Signal with Calretinin Antibody
(1)
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MilliporeSigma
December 19, 2022
This antibody was used in mice P12 brain sections to determine the subset of interneurons positive for Calretinin.
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Good Poly(vinyl alcohol) for Electrospinning
(1)
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MilliporeSigma
December 08, 2022
I use PVA primarily for the production of a nanofibrous membrane. The nanofibrous membrane is prepared using the electrospinning technique ...
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Poloxamer Used for Thermo Responsive Hydrogel
(1)
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MilliporeSigma
December 06, 2022
Pluronic F-127 is an interesting Poloxamer that I have been using for the fabrication of a thermo responsive hydrogel. The sol-gel ...
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Good Product for Biomimetic Systems
(1)
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MilliporeSigma
December 06, 2022
I used chitosan medium molecular weight for its innate antimicrobial properties. I usually blend it with another biocompatible polymer to ...
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Useful Reagent for Chemical Synthesis
(1)
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MilliporeSigma
December 06, 2022
I use triethylamine in few step of a chemical synthesis where I need to add amine groups to another product in order to finally obtain a ...
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Good Product for Crosslinking
(1)
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MilliporeSigma
December 05, 2022
I am developing a functionalized membrane for the treatment of wound healing. NHS helps me obtaining the crosslinking of proteins together ...
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Good Product for Electrospinning Applications
(1)
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MilliporeSigma
December 05, 2022
I use Poly(ethylene oxide) (PEO) as a copolymer in formulation used for electrospinning. Thanks to PEO I am able to create nanofibrous ...
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Great Astrocyte Marker
(1)
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MilliporeSigma
December 02, 2022
I have used this antibody to detect astrocyte among a number of neurons within the mouse brain. It has worked very well in mouse sample.
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Excellent One-Step qPCR Kit
(1)
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MilliporeSigma
November 30, 2022
Our group focuses on the utilization of CD8 T-cells as means of cancer treatment. For our experimental purposes and the development of such ...
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Great Labelling of Acetylcholine Neurons
(1)
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MilliporeSigma
November 29, 2022
I have used this antibody to detect acetylcholine neurons from the mouse striatum. It has worked greatly in mouse sample.
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Antibody Validated for MAFb ChIP Experiments
(1)
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MilliporeSigma
November 17, 2022
MAFB is an important transcription factor for different cell type. In the kidney it is crucial for the differentiation and adult homeostasis...
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