Buffers for use in SDS-PAGE are available from several suppliers. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) is an analytical technique used to separate proteins based on their molecular weight. Loading buffers are important when preparing samples to be loaded into the gel for electrophoresis. Loading buffers can vary in composition but often contain Tris-HCL, SDS, Bromophenol blue, and glycerol. SDS denatures proteins and adds a net negative charge for movement in an electric field. Bromophenol blue is used to visualize the run of the proteins on the gel. Glycerol increases the density of the sample to make loading easier and Tris helps to maintain the pH. ß-mercaptoethanol or dithiothreitol (DTT) can also be added to the buffer to break disulfide bonds and maintain the unfolded state. Varying buffer concentrations such as 2X, 3X, 4X are available. Visit the supplier page for more product information.
Your search returned 34 SDS-PAGE Loading Buffers across 10 suppliers.
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MyBioSource.com
- On the basis of bromophenol blue dye, SDS-PAGE Protein Loading Buffer is a kind of 5X concentrated solution, it contains...
- 3 ml
- Western Blot
- SDS-PAGE Protein Sample Loading Buffer (5X) provides a simple and convenient method for the loading of conventional ...
- 10 mL
- Inquire
Cell Signaling Technology
New England Biolabs
- Blue Loading Buffer Reagents for SDS-PAGE:
3X SDS Blue Loading Buffer (8 ml):
187.5 mM Tris-HCl (pH 6.8 @ 25°C), 6% (... - 8 ml
- Proteomics & Glycomics
Read More
- Gel Loading Dye, Blue (6X) is a pre-mixed loading buffer with one tracking dye for agarose and non-denaturing ...
- 4 ml
- DNA Manipulation
LICORbio
- 4X Protein Sample Loading Buffer is optimized for use as a loading buffer for protein gel electrophoresis. This orange ...
- 15 mL
- Inquire
- 4X Protein Sample Loading Buffer is optimized for use as a loading buffer for protein gel electrophoresis. This orange-...
- 1 kit
- Inquire
Abcam
- 10 ml
- SDS-PAGE, Western blot
BioLegend
- Western-Ready(TM) Protein Sample Loading Buffer (5X)
- 20 mL
- Western Blot (WB)
Advansta
- Pre-mixed loading buffers for protein electrophoresis
- 1ml
- Protein electrophoresis
- Pre-mixed loading buffers for protein electrophoresis
- 5ml
- Protein electrophoresis
BosterBio
- SDS PAGE Sample Buffer 2X (Reducing) is the most commonly used sample buffer for Sodium Dodecyl Sulfate - Polyacrylamide...
- 20ml
- Inquire
- SDS-PAGE Protein Loading Buffer 5X (Reducing)
- From 3mL to 20mL
- Inquire
Abbexa Ltd
- SDS-PAGE Sample Loading Buffer is a 2X concentrated solution based on Bromophenol blue dye for use in SDS-PAGE protein ...
- 5 ml
- WB
- SDS-PAGE Sample Loading Buffer is a 5X concentrated solution based on Bromophenol blue dye for use in SDS-PAGE protein ...
- 5 ml, 100 ml
- WB
Thermo Fisher Scientific
- Compatible with denaturing polyacrylamide gels
A 1–2X solution of 95% Formamide, 18 mM EDTA, and 0.025% SDS, Xylene... - 1 mL
- Inquire
- Compatible with denaturing polyacrylamide gels
A 1–2X solution of 95% Formamide, 18 mM EDTA, and 0.025% SDS, Xylene... - 10 mL
- Inquire
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