4X Protein Sample Loading Buffer, 15 mL from LICORbio

Product Specs
Item4X Protein Sample Loading Buffer, 15 mL
CompanyLICORbio
Price Pricing Info Supplier Page View Company Product Page
Catalog Number928-40004
Quantity15 mL
Brand/TrademarkLI-COR Biosciences

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LICORbio

4647 Superior

St. Lincoln, NE 68504

United States

Phone: 800-645-4267
402-467-0700

Company Profile

Website: Website: www.licor.com/bio

Website: Reagents: www.licor.com/bio/reagents

Website: Special offers: www.licor.com/bio/special-offers

(1) ORF3c is expressed in SARS‐CoV‐2‐infected cells and inhibits innate sensing by targeting MAVSEMBO reportsDecember 6, 2023Martin Müller, Alexandra Herrmann, Shigeru Fujita, Keiya Uriu, Carolin Kruth, Adam Strange, Jan E Kolberg, Markus Schneider, Jumpei Ito, Marcel A Müller, et al.at 20,800 g for 20 min at 4°C. Lysates were mixed with Protein Sample Loading Buffer (LI‐COR, Cat# 928‐40004) supplemented with 10% β‐mercaptoethanol and heated at 95°C for 5 min. Proteins were separated on NuPAGE

(2) NG2/CSPG4 attenuates motility in mandibular fibrochondrocytes under serum starvation conditionsFrontiers in Cell and Developmental BiologyNovember 7, 2023Shin Young Ahn, Mina Bagheri Varzaneh, Yan Zhao, Jacob Rozynek, Sriram Ravindran, Jonathan Banks, Minahil Chaudhry, David A. Reedfor 3 h at 37°C. For all samples, the lysates were adjusted to a 4x Protein Sample Loading Buffer (928–40004, LI-COR, Lincoln, NE), heated at 100°C for 5 min, run on a 4%–15% sodium dodecyl sulfate polyacrylamide

(3) Doxorubicin-induced modulation of TGF-β signaling cascade in mouse fibroblasts: insights into cardiotoxicity mechanismsScientific ReportsNovember 2, 2023Conner Patricelli, Parker Lehmann, Julia Thom Oxford, Xinzhu Pu(Thermo Fisher Scientific; 23225). Protein samples (30 μg) were prepared with 4X protein sample loading buffer (LI-COR; 928-40004), and loaded onto 4–12% NuPAGE™ Bis–Tris gels (Thermo Fisher Scientific; NP0321BOX). The proteins were

(4) Pluripotent stem cell-derived neural progenitor cells can be used to model effects of IL-6 on human neurodevelopmentDisease Models & MechanismsNovember 1, 2023Kseniia Sarieva, Felix Hildebrand, Theresa Kagermeier, Zeynep Yentür, Katharina Becker, Simone Mayer(Thermo Fisher Scientific, 23227). Proteins were denatured by boiling with Protein Sample Loading Buffer (Li-COR, 928-40004) at 95°C for 5 min. Then, 10 or 15 µg of protein was loaded in 4-15% Mini-PROTEAN TGX Precast Gels (Bio-Rad,

(5) Parabens Promote Protumorigenic Effects in Luminal Breast Cancer Cell Lines With Diverse Genetic AncestryJournal of the Endocrine SocietyJuly 3, 2023Jazma L Tapia, Jillian C McDonough, Emily L Cauble, Cesar G Gonzalez, Dede K Teteh, Lindsey S Treviño(Thermo Scientific, CAT: 23227). Lysate mixed (10-25 μg) with 4 times sample loading buffer (Licor, CAT: 928-40004) and Novex 10X bolt sample reducing agent (Invitrogen, CAT: B0009) were loaded per lane. The proteins

(6) A rapid cell-free expression and screening platform for antibody discoveryNature communicationsJuly 3, 2023Hunt AC, Vögeli B, Hassan AO, Guerrero L, Kightlinger W, Yoesep DJ, Krüger A, DeWinter M, Diamond MS, Karim AS, et al.37 °C for 10 min. 3 μL of the CFPS and RNAseA mixture were combined with 4x loading buffer (LiCor, 928-40004) and the samples were subsequently denatured at 70 °C for 3 min, then separated via SDS-PAGE and imaged

(7) SMYD5 is a novel epigenetic gatekeeper of the mild hypothermia responsebioRxiv : the preprint server for biologyJune 9, 2023Rafnsdottir S, Jang K, Halldorsdottir ST, Tomasdottir A, Vinod M, Möller K, Reynisdottir T, Atladottir LH, Allison KE, He J, et al.4°C and the supernatant was collected. The supernatant was diluted with 4X loading buffer (LI-COR, 928–40004) and heated at 95°C for 5 min. The protein concentration was measured using Pierce™ BCA Assay Kit (Thermo Fisher Scientific

(8) The mitochondrial Ca2+ channel MCU is critical for tumor growth by supporting cell cycle progression and proliferationFrontiers in Cell and Developmental BiologyJune 8, 2023Emily Fernandez Garcia, Usha Paudel, Michael C. Noji, Caitlyn E. Bowman, Anil K. Rustgi, Jason R. Pitarresi, Kathryn E. Wellen, Zolt Arany, Jillian S. Weissenrieder, J. Kevin Foskett(Thermo Scientific, 23227). Samples were prepared for loading with 4X LICOR loading buffer (LICOR, 928-40004) and β-mercaptoethanol. Samples were boiled at 100°C for 5 min. Gel electrophoresis was performed in

(9) Microenvironmental mechanoactivation through Yap/Taz suppresses chondrogenic gene expressionMolecular Biology of the CellJune 1, 2023Grey F. Hallström, Dakota L. Jones, Ryan C. Locke, Edward D. Bonnevie, Sung Yeon Kim, Lorielle Laforest, Diana Cruz Garcia, Robert L. Mauckused as a standard. WCL was denatured and reduced using 4X Li-COR sample buffer (Li-COR Biosciences; 928-40004) with β-mercaptoethanol and boiling at 95°C for 5 min. A total of 25 μg of protein was loaded per lane

(10) Inhibition of Mitochondrial Antioxidant Defense and CDK4/6 in MesotheliomaMoleculesMay 27, 2023Marian Kratzke, George Scaria, Stephen Porter, Betsy Kren, Mark A. KleinDirect Detect Spectrometer. Twenty micrograms of protein were mixed with LI-COR (Lincoln, NE, USA) Loading Buffer (catalog 928–40004) containing 0.05 M DTT and heated at 95 °C for 5 min. Samples were subjected to electrophoresis on 4–20%

(11) Disrupting mechanical homeostasis promotes matrix metalloproteinase-13 mediated processing of neuron glial antigen 2 in mandibular condylar cartilageEuropean Cells and MaterialsMay 8, 2023M. Bagheri Bagheri Varzaneh, Y. Zhao, J. Rozynek, M. Han, D.A. Reedlonger saturated. All filter paper samples were placed directly into 4 × Protein Sample Loading Buffer (928-40004, LI-COR, Lincoln, NE, USA), spun down on a desktop centrifuge to remove red blood cells, then the supernatant

(12) The mitochondrial Ca 2+ channel MCU is critical for tumor growth by supporting cell cycle progression and proliferationbioRxivMay 3, 2023Emily Fernández García, Usha Paudel, Michael C. Noji, Caitlyn E. Bowman, Jason R. Pitarresi, Anil K. Rustgi, Kathryn E. Wellen, Zolt Arany, Jillian S. Weissenrieder, J. Kevin Foskett(Thermo Scientific, 23227). Samples were prepared for loading with 4X LICOR loading buffer (LICOR, 928-40004) and β-mercaptoethanol. Samples were boiled at 100°C for 5 min. Gel electrophoresis was performed in

(13) Thymidine phosphorylase facilitates retinoic acid inducible gene-I induced endothelial dysfunctionCell Death & DiseaseApril 26, 2023Adrian Baris, Eugenia Fraile-Bethencourt, Jaiden Eubanks, Sokchea Khou, Sudarshan Anand23227, Thermofisher). For western blot, samples were mixed with 4x Protein Sample Loading Buffer (Cat: 928-40004, Li-Cor) supplemented with 5% of beta-mercaptoethanol, denaturalized and loaded in 4–20% precast polyacrylamide

(14) Dissecting the influence of cellular senescence on cell mechanics and extracellular matrix formation in vitroAging CellMarch 1, 2023Erik Brauer, Tobias Lange, Daniela Keller, Sophie Görlitz, Simone Cho, Jacqueline Keye, Manfred Gossen, Ansgar Petersen, Uwe Kornak4906845001; Roche) according to manufacturer's instructions. Lysates were mixed with 4× Loading Buffer (928‐40004, Li‐Cor) and denatured at 95°C for 5 min prior to electrophoresis. SDS‐PAGE was performed with the NuPAGE®

(15) NG2/CSPG4 regulates cartilage degeneration during TMJ osteoarthritisFrontiers in Dental MedicineJanuary 23, 2023David A. Reed, Yan Zhao, Mina Bagheri Varzaneh, Jun Soo Shin, Jacob Rozynek, Michael Miloro, Michael Hanunits/ml for 2 h at 37 °C. For all samples, lysates were adjusted to a 1× Protein Sample Loading Buffer (928-40004, Licor, Lincoln, NE), heated at 99 °C for 10 min, separated on a 4%–15% sodium dodecyl sulfate polyacrylamide

(16) Arc ubiquitination regulates endoplasmic reticulum-mediated Ca(2+) release and CaMKII signalingFrontiers in cellular neuroscienceJanuary 1, 2023Ghane MA, Wei W, Yakout DW, Allen ZD, Miller CL, Dong B, Yang JJ, Fang N, Mabb AM.were washed with Co-IP buffer and proteins were eluted from beads using SDS sample buffer (Li-Cor #928-40004) with heating at 45°C for 5 min. Subsequently, beads were pelleted by centrifugation (15,000 rpm for

(17) Desmin intermediate filaments and tubulin detyrosination stabilize growing microtubules in the cardiomyocyteBasic Res CardiolNovember 3, 2022Alexander K. Salomon, Sai Aung Phyo, Naima Okami, Julie Heffler, Patrick Robison, Alexey I. Bogush, Benjamin L. ProsserSignaling #5872S) on ice for 1 h. The supernatant was collected and combined with 4X loading dye (Li-COR #928-40004), supplemented with 10% 2-mercaptoethanol, and boiled for 10 min. The resulting lysate was resolved

(18) Identification and targeting of a HES1â€YAP1â€CDKN1C functional interaction in fusionâ€negative rhabdomyosarcomaMolecular OncologyOctober 1, 2022Alexander R. Kovach, Kristianne M. Oristian, David G. Kirsch, Rex C. Bentley, Changde Cheng, Xiang Chen, Poâ€Han Chen, Jenâ€Tsan Ashley Chi, Corinne M. LinardicSpectraMax ABS instrument. Protein concentrations were normalized and mixed with 4× loading dye (LI‐COR #928‐40004, LI‐COR Biosciences, Lincoln, NE, USA or BioRad #1610747). Thirty to fifty micrograms total protein

(19) Deletion of a previously uncharacterized lipoprotein lirL confers resistance to an inhibitor of type II signal peptidase in Acinetobacter baumanniiProceedings of the National Academy of SciencesSeptember 20, 2022Ke-Jung Huang et al.lysonase (Novagen). Cell lysates were mixed with equivalent volume of 2× SDS sample buffer (LI-COR 928-40004 containing 5% 2-mercaptoethanol) and heated at 95 °C for 15 min before loading. Detailed methods are

(20) Determinants of Spike infectivity, processing, and neutralization in SARS-CoV-2 Omicron subvariants BA.1 and BA.2Cell Host & MicrobeSeptember 14, 2022Chiara Pastorio, Fabian Zech, Sabrina Noettger, Christoph Jung, Timo Jacob, Theo Sanderson, Konstantin M.J. Sparrer, Frank Kirchhoff(PEI),Sigma-Aldrich,Cat#408727-100ML 4 % Paraformaldehyde (PFA),ChemCruz,Cat#sc-281692 4X Protein Sample Loading Buffer,LI-COR,Cat#928-40004 Glycerol,Sigma-Aldrich,Cat#G5516-500ML Mowiol 4-88,ROTH,Cat#0713.1 Tween 20,Sigma-Aldrich,Cat#P7949-500ML Tris-Cl,Sigma-Aldrich,Cat#T5941-500G "DABCO

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4X Protein Sample Loading Buffer, 15 mL from LICORbio

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4X Protein Sample Loading Buffer, 15 mL from LICORbio

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