Description
Blue Loading Buffer Reagents for SDS-PAGE:
3X SDS Blue Loading Buffer (8 ml):
187.5 mM Tris-HCl (pH 6.8 @ 25°C), 6% (w/v) SDS, 30% glycerol and 0.03% (w/v) bromophenol blue. (Store at room temperature)
30X Reducing Agent (1 ml): 1.25 M DTT (Store at -20°C.)