| Silencer™ Gene Specific siRNAs
and Negative Controls • GAPDH, c-myc, cyclophilin, and scrambled siRNA controls • Available as ready to use siRNA and as Silencer™siRNA Construction Kit templates • Functionally tested in several common cell lines Whether you are starting out in siRNA research or you are an siRNA expert, you'll appreciate the convenience of the Silencer™ gene specific siRNA controls. These controls are ideal for developing and optimizing your siRNA experiments and are validated for use in mouse, rat, and human cell lines. Each Silencer gene specific siRNA control contains 50 µg of ready to use chemically synthesized GAPDH, cyclophilin, or c-myc siRNA, which is sufficient for 165 transfections (24 well plates). A scrambled Negative Control siRNA (25 µg) is also included. For scientists who are already using the Silencer siRNA Construction Kit to produce their siRNA, Ambion recommends using the Silencer GAPDH, Cyclophilin, or c-myc siRNA Templates. These templates, when used in conjunction with The Silencer siRNA Construction Kit, produce siRNAs corresponding to Ambion's GAPDH, Cyclophilin, c-myc, and scrambled siRNA control sequences (described above). Ambion has also designed and tested three Negative Control siRNAs that have no significant homology to mouse, rat, or human gene sequences. The Negative Control siRNA #1, #2, and #3 are ideal for use in any siRNA experiment as controls for nonspecific effects on gene expression. Three controls are offered in the unlikely event that one or another has an unanticipated effect on the expression of your gene. Ambion's Negative Control siRNAs are available as ready to use chemically synthesized siRNA or as DNA templates for use with the Silencer siRNA Construction Kit. |