RIPA Buffer (10X) from Cell Signaling Technology

Product Specs
ItemRIPA Buffer (10X)
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number9806S
Quantity15 ml
Research AreaRelated Products
ApplicationsW

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

Website: Technical Support

(1) Synthesis and Preclinical Evaluation of 22-[18F]Fluorodocosahexaenoic Acid as a Positron Emission Tomography Probe for Monitoring Brain Docosahexaenoic Acid Uptake KineticsACS Chem NeurosciDecember 20, 2023Marlon Vincent V. Duro, Juno Van Valkenburgh, Diana E. Ingles, Jenny Tran, Zhiheng Cai, Brandon Ebright, Shaowei Wang, Bilal E. Kerman, Jasmin Galvan, Sung Hee Hwang, et al.or 15 μM) for 6 h. Cells were then lysed in 60 μL of 1× RIPA buffer (Cell Signaling Technology, CST 9806) containing a protease inhibitor cocktail (Sigma, P8340), phosphatase inhibitor cocktail (Sigma, P0044),

(2) Combining the Tyrosine Kinase Inhibitor Cabozantinib and the mTORC1/2 Inhibitor Sapanisertib Blocks ERK Pathway Activity and Suppresses Tumor Growth in Renal Cell CarcinomaCancer ResDecember 15, 2023Yige Wu, Siqi Chen, Xiaolu Yang, Kazuhito Sato, Preet Lal, Yuefan Wang, Andrew T. Shinkle, Michael C. Wendl, Tina M. Primeau, Yanyan Zhao, et al.later use. Immunoblotting Flash-frozen tissues were washed with 1× PBS, submerged in RIPA buffer [#9806, Cell Signaling Technology (CST)] supplemented with protease (#05892791001) and phosphatase (#04906837001)

(3) An ex vivo model of interactions between extracellular vesicles and peripheral mononuclear blood cells in whole bloodJ Extracell VesiclesDecember 4, 2023Blanca V. Rodriguez, Yi Wen, Erin N. Shirk, Samuel Vazquez, Olesia Gololobova, Amanda Maxwell, Jessica Plunkard, Natalie Castell, Bess Carlson, Suzanne E. Queen, et al.were lysed in 1x radioimmunoprecipitation assay buffer (RIPA, Cell Signalling Technology, catalog #9806) for 30 min at room temperature. Lysates were heated at 95˚C for 5 min together with Laemmli sample buffer

(4) The role of apoptosis and oxidative stress in the pathophysiology of Acanthamoeba spp. infection in the kidneys of hosts with different immunological statusParasit VectorsDecember 1, 2023Karolina Kot, Patrycja Kupnicka, Maciej Tarnowski, Patrycja Tomasiak, Danuta Kosik-Bogacka, Natalia Łanocha-Arendarczykgel) layer. Samples were lysed in RIPA buffer (Cell Signaling Technology, Danvers, MA, USA [cat. no.: 9806]) with protease and phosphatase inhibitors (PhosSTOP™; Roche, Basel, Switzerland [cat. no.: 4906845001];

(5) The subset of peroxisomal tail-anchored proteins do not reach peroxisomes via ER, instead mitochondria can be involvedPLoS OneDecember 1, 2023Tamara Somborac, Güleycan Lutfullahoglu Bal, Kaneez Fatima, Helena Vihinen, Anja Paatero, Eija Jokitalo, Ville O. Paavilainen, Svetlana Konovalova50 cells were analyzed. Immunoblotting Cells were washed with PBS and lysed in RIPA buffer (CST, 9806). Protein concentration was determined by BCA assay kit (Thermo Scientific, 23227). Protein lysates

(6) PRR14 organizes H3K9me3-modified heterochromatin at the nuclear laminaNucleus (Austin, Tex.)December 1, 2023Kiseleva AA, Cheng YC, Smith CL, Katz RA, Poleshko A.condition. Western blotting Cell lysates were prepared using 1x RIPA buffer (Cell Signaling, cat# 9806) supplemented with 1% SDS, 1x Halt™ Protease and Phosphatase Inhibitor (ThermoFisher Scientific, cat#

(7) Lipolysis supports bone formation by providing osteoblasts with endogenous fatty acid substrates to maintain bioenergetic statusBone ResearchNovember 24, 2023Ananya Nandy, Ron C. M. Helderman, Santosh Thapa, Shobana Jayapalan, Alison Richards, Nikita Narayani, Michael P. Czech, Clifford J. Rosen, Elizabeth Rendina-Ruedyprotein. Protein was isolated from cells by lysing the cells with 1× RIPA (Cell Signaling Technology; 9806) buffer in the presence of protease inhibitor cocktail (Roche; 04693116001) and phosphatase inhibitor

(8) WIP1 is a novel specific target for growth hormone actioniScienceNovember 17, 2023Tugce Apaydin, Svetlana Zonis, Cuiqi Zhou, Christian Wong Valencia, Robert Barrett, Ger J. Strous, Jan A. Mol, Vera Chesnokova, Shlomo Melmed,ThermoFisher Scientific,Cat# 78440 Protease inhibitor cocktail,MilliporeSigma,Cat# P8340 RIPA buffer,Cell Signaling,Cat #9806S Polybrene,Santa Cruz Biotechnology,Cat# sc-134220 Trizol,Invitrogen,Cat# 15596018 Histopaque 1077

(9) A Massively Parallel Screen of 5′UTR Mutations Identifies Variants Impacting Translation and Protein Production in Neurodevelopmental Disorder GenesmedRxivNovember 9, 2023Stephen P. Plassmeyer, Colin P. Florian, Michael J. Kasper, Rebecca Chase, Shayna Mueller, Yating Liu, Kelli McFarland White, Courtney F. Jungers, Slavica Pavlovic Djuranovic, Sergej Djuranovic, et al.Supernatant was extracted and discarded. Lysis buffer consisting of 10x RIPA Buffer (Cell Signaling, 9806) diluted to 1x with water and 50x proteinase inhibitor diluted to 1x was added to pelleted cells. Cells

(10) Pluripotent stem cell-derived neural progenitor cells can be used to model effects of IL-6 on human neurodevelopmentDisease Models & MechanismsNovember 1, 2023Kseniia Sarieva, Felix Hildebrand, Theresa Kagermeier, Zeynep Yentür, Katharina Becker, Simone Mayerprotein extraction was performed by collecting the cells in 1× RIPA buffer (Cell Signaling Technology, 9806) supplemented with 1× Halt protease and phosphatase inhibitor (Thermo Fisher Scientific, 78443) on ice.

(11) A protein complex of LCN2, LOXL2 and MMP9 facilitates tumour metastasis in oesophageal cancerMolecular OncologyNovember 1, 2023Qiaoxi Xia, Zepeng Du, Mantong Chen, Xiao Zhou, Wenjing Bai, Xiaoqi Zheng, Ling Lin, Yan Zhao, Jiyu Ding, Zhisheng Wu, et al.transfection and then starved for 12 h in serum‐free medium. Cells were then lysed in RIPA buffer (9806S, CST, Danvers, MA, USA) to extract the total protein. Conditioned medium was concentrated using Amicon® Ultra centrifugal filters

(12) Episodic live imaging of cone photoreceptor maturation in GNAT2-EGFP retinal organoidsDisease Models & MechanismsNovember 1, 2023Jinlun Bai, David S. Koos, Kayla Stepanian, Zachary Fouladian, Dominic W. H. Shayler, Jennifer G. Aparicio, Scott E. Fraser, Rex A. Moats, David Cobrinikdissociation. Dissociated ROs were washed with PBS, lysed with RIPA buffer (Cell Signaling Technology, 9806S) plus a protease inhibitor cocktail (Sigma-Aldrich, 5892970001) for 1 h on ice, and centrifuged at 15,000

(13) Rheumatoid Synovial Fluid and Acidic Extracellular pH Modulate the Immunomodulatory Activity of Urine-Derived Stem CellsInternational Journal of Molecular SciencesNovember 1, 2023Michaela Cehakova, Dana Ivanisova, Magdalena Strecanska, Jana Plava, Zuzana Varchulova Novakova, Andreas Nicodemou, Stefan Harsanyi, Martina Culenova, Sona Bernatova, Lubos Danisovicdescribed in Section 2.5. Post-incubation UdSCs were washed with ice-cold PBS and lysed in RIPA Buffer (9806S, Cell Signaling, Danvers, MA, USA) supplemented with Complete Protease Inhibitors (Roche, Basel, Germany)

(14) Thiobarbiturate-Derived Compound MHY1025 Alleviates Renal Fibrosis by Modulating Oxidative Stress, Epithelial Inflammation, and Fibroblast ActivationAntioxidants (Basel)October 31, 2023Jeongwon Kim, Jieun Lee, Dahye Yoon, Minjung Son, Mi-Jeong Kim, Sugyeong Ha, Doyeon Kim, Ji-an Yoo, Donghwan Kim, Hae Young Chung, et al.total protein lysates from the tissues. Total protein was extracted from cells using RIPA buffer (#9806, Cell Signaling Technology, Danvers, MA, USA) containing a protease inhibitor cocktail. Throughout the

(15) Repaglinide Induces ATF6 Processing and Neuroprotection in Transgenic SOD1G93A MiceInternational Journal of Molecular SciencesOctober 30, 2023Rafael Gonzalo-Gobernado, Laura Moreno-Martínez, Paz González, Xose Manuel Dopazo, Ana Cristina Calvo, Isabel Pidal-Ladrón de Guevara, Elisa Seisdedos, Rodrigo Díaz-Muñoz, Britt Mellström, Rosario Osta, et al.preparation was performed as described [93]. In brief, tissue was homogenized on ice in RIPA buffer (9806, Cell Signaling Technology, Danvers, MA, USA) supplemented with protease inhibitor (Complete EDTA-free,

(16) Anti-inflammatory and anti-fibrotic effects of berberine-loaded liquid crystalline nanoparticlesEXCLI JOctober 26, 2023Amlan Chakraborty, Keshav Raj Paudel, Chao Wang, Gabriele De Rubis, Dinesh Kumar Chellappan, Philip Michael Hansbro, Chrishan S. Samuel, Kamal Duaprotein extraction using 20 μl of a cocktail of 1X RIPA lysis buffer (from 10X RIPA, Cell signalling, catalogue no. 9806) with phosphatase/protease inhibitor (Cell signalling, catalogue no. 5872) and PMSF (Cell signalling,

(17) Rutin alleviates ventilator-induced lung injury by inhibiting NLRP3 inflammasome activationiScienceOctober 20, 2023Shengsong Chen, Yu Bai, Jingen Xia, Yi Zhang, Qingyuan ZhanApoptosis Assay Kit,Roche,11684817910 DAPI,Abcam,ab104139 RIPA lysis buffer,Cell Signaling Technology,Cat#9806 protease inhibitor,Sigma,539131 bicinchoninic acid (BCA) protein assay kit,Thermo Fisher Scientific,23227 chemiluminescent

(18) Pooled endogenous protein tagging and recruitment for scalable discovery of effectors for induced proximity therapeuticsResearch SquareOctober 18, 2023Yevgeniy V. Serebrenik, Yevgeniy Serebrenik, Timothé Maujean, George M. Maujean, George Burslem1:10,000). Immunoblotting Cultured cells were pelleted, washed with PBS, and resuspended in RIPA lysis buffer (Cell Signaling, 9806) with 1x protease inhibitor cocktail (MilliporeSigma, P8340). Samples were normalized by bicinchoninic

(19) Tolfenamic Acid Derivatives: A New Class of Transcriptional Modulators with Potential Therapeutic Applications for Alzheimer’s Disease and Related DisordersInternational Journal of Molecular SciencesOctober 16, 2023Jaunetta Hill, Karim E. Shalaby, Syed W. Bihaqi, Bothaina H. Alansi, Benjamin Barlock, Keykavous Parang, Richard Thompson, Khalid Ouararhni, Nasser H. Zawiawashed using 1X PBS and then lysed using 1X radio-immunoprecipitation assay (RIPA) lysis buffer (Cat. 9806S, Cell Signaling Technology, Danvers, MA, USA) supplemented with a phosphatase and protease inhibitor

(20) SEPTIN 7 INTERACTS WITH NUMB TO PRESERVE SARCOMERE STRUCTURAL ORGANIZATION AND MUSCLE CONTRACTILE FUNCTIONbioRxiv : the preprint server for biologyOctober 16, 2023De Gasperi R, Csernoch L, Dienes B, Gonczi M, Chakrabarty JK, Goeta S, Aslan A, Toro CA, Karasik D, Brown LM, et al.(TA) and extensor digitorum longus (EDL) were homogenized using an MP homogenizer in RIPA buffer (#9806, Cell Signaling Technologies Inc., Danvers, MA) supplemented with a protease and phosphatase inhibitor

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5/10/2022Great Protein Extraction Buffer Owen JiangThe nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the immune system – the mammalian defense to challenges against ... Read Review

RIPA Buffer (10X) from Cell Signaling Technology

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RIPA Buffer (10X) from Cell Signaling Technology

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