Cell Signaling Techonology
RIPA Buffer (10X)
#9806
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The nutritional composition of diet fundamentally modulates core physiological systems, including whole-body metabolic programs and the immune system – the mammalian defense to challenges against homeostasis. Ketogenic diets (KDs) are extremely high in fats and very low in carbohydrates (calories 90% from fat, 10% from protein, and less than 0.1% from carbohydrates) that rewire whole-body metabolism. This metabolic adaptation improves many physiological processes including aging, thermogenesis, and glucose homeostasis. However, the effects of KDs on the immune system remain less explored. Thus, the overall objective of my PhD studies will be to elucidate how diets, particularly KD, affect the immune response in adaptive and maladaptive contexts.
Protein extraction
Cell or mouse tissues
Protease inhibitors and phosphatase inhibitor could be added to prevent protein phosphorylation loss.
1X RIPA buffer can be used to lyse cell culture or bulk mouse tissue. For cells, gentle scraping is sufficient to lyse the cells. For tissues, homogenizers are commended. After high-speed spinning, protein extraction will be in the top aqueous layer.
N/A
Reliable and affordable protein extraction buffer.
Great product for downstream western blot assays.