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Protein Biochemistry
Protein Purification
Lysis Buffers / Cell Lysis Kits
Cell Lysis Buffer (10X)
Cell Lysis Buffer (10X) from Cell Signaling Technology
(1)
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Citations:
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Supplier Page
Supplier Page from
Cell Signaling Technology for
Cell Lysis Buffer (10X)
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Citations
Reviews
Product Specs
Item
Cell Lysis Buffer (10X)
Company
Cell Signaling Technology
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
9803S
Quantity
15 ml
Research Area
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Cell Signaling Technology
3 Trask Lane
Danvers
,
Massachusetts
01923
United States
Phone
: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488
Company Profile
Website:
http://www.cellsignal.com/
Website:
Technical Support
Citations (1233)
(1)
Beta cell primary cilia mediate somatostatin responsiveness via SSTR3
Islets
December 31, 2023
Samantha E. Adamson, Zipeng A. Li, Jing W. Hughes
sodium pyrophosphate, 1 mM beta-glycerophosphate, 1 mM Na3VO4, 1 µg/ml leupeptin,
Cell Signaling
, #
9803
) with protease
inhibitor
(Roche, #11836170001) for 30 min on ice with intermittent vortexing. Lysates
(2)
Inhibition of LRRK2 kinase activity rescues deficits in striatal dopamine physiology in VPS35 p.D620N knock-in mice
NPJ Parkinsons Dis
December 18, 2023
Mengfei Bu, Jordan Follett, Isaac Deng, Igor Tatarnikov, Shannon Wall, Dylan Guenther, Melissa Maczis, Genevieve Wimsatt, Austen Milnerwood, Mark S. Moehle,
et al.
immunoblotting
.
Immunoblotting
Striatal tissues were homogenized and lysed in 1x Lysis Buffer (
Cell Signaling
#
9803
S) with 1 mM
PMSF
and 1X phosphatase
inhibitors
(
Thermofisher
; #
36978
and #
78427
, respectively). Homogenates
(3)
Rho GTPase activity crosstalk mediated by Arhgef11 and Arhgef12 coordinates cell protrusion-retraction cycles
Nat Commun
December 15, 2023
Suchet Nanda, Abram Calderon, Arya Sachan, Thanh-Thuy Duong, Johannes Koch, Xiaoyi Xin, Djamschid Solouk-Stahlberg, Yao-Wen Wu, Perihan Nalbant, Leif Dehmelt
Western blot analysis
Cells were washed one time with ice cold PBS, then lysed with ice cold 1×
cell lysis
buffer (
9803
,
CST
) for 5 min on ice. The cell lysate was then centrifuged at 17000 × g for 10 min at 4 °C to remove
(4)
Critical shifts in lipid metabolism promote megakaryocyte differentiation and proplatelet formation
Nature Cardiovascular Research
December 11, 2023
Bianca de Jonckheere, Ferdinand Kollotzek, Patrick Münzer, Vanessa Göb, Melina Fischer, Kristina Mott, Cristina Coman, Nina Nicole Troppmair, Mailin-Christin Manke, Monika Zdanyte,
et al.
Aldrich P8920-100ML, 0.1%), paraformaldehyde (PFA) (Fisher 6131108),
cell lysis
buffer (
Cell Signaling
9803S
), protease and phosphatase
inhibitor
cocktail (
Cell signaling
5872S
),
FcR blocking reagent mouse
(
Miltenyi Biotec
(5)
Loss of Slc35a2 alters development of the mouse cerebral cortex
bioRxiv
December 1, 2023
Soad Elziny, Sahibjot Sran, Hyojung Yoon, Rachel R. Corrigan, John Page, Amanda Ringland, Anna Lanier, Sara Lapidus, James Foreman, Erin L. Heinzen,
et al.
homogenized with a pestle homogenizer in the following solution: 1x Cell Lysis Buffer (
Cell Signaling
,
9803S
), 0.5% (v/v) Dithiothreitol (
ThermoFisher Scientific
,
327190010
), 1% (v/v)
PMSF
(
Cell Signaling
,
8553
),
(6)
AMP-activated protein kinase is necessary for Treg cell functional adaptation to microenvironmental stress
bioRxiv
December 1, 2023
Manuel A. Torres Acosta, Nurbek Mambetsariev, Carla P Reyes Reyes Flores, Kathryn A. Helmin, Qianli Liu, Anthony M. Joudi, Luisa Morales-Nebreda, Jonathan Gurkan, Kathleen Cheng, Hiam Abdala-Valencia,
et al.
(33).
Western blotting
.
Cultured cells
were lysed for one hour at 4°C in lysis buffer (
Cell Signaling
cat. no.
9803
) supplemented with phosphatase (
Cell Signaling
cat. no.
5870S
) and
protease inhibitors
(Roche, cat.
(7)
The synthetic oleanane triterpenoid CDDO-2P-Im binds GRP78/BiP to induce unfolded protein response-mediated apoptosis in myeloma
Molecular oncology
December 1, 2023
Luo G, Aldridge K, Chen T, Aslot V, Kim BG, Han EH, Singh N, Li S, Xiao TS, Sporn MB,
et al
.
sacrificed 12 h later for analyses of
tumors
.
Tumors
were lysed for protein using Cell Lysis solution (#
9803
;
Cell Signaling
, Danvers, MA, USA) and subsequently prepared for
immunoblot
. Female and Male NSG (NOD
(8)
Regulated dynamic subcellular GLUT4 localization revealed by proximal proteome mapping in human muscle cells
J Cell Sci
December 1, 2023
Anuttoma Ray, Jennifer Wen, Lucie Yammine, Jeff Culver, Isabella Supardi Parida, Jeonifer Garren, Liang Xue, Katherine Hales, Qing Xiang, Morris J. Birnbaum,
et al.
Western blot analysis
Proteins were extracted from 3-day differentiated SKM cells using
cell lysis
buffer (
9803S
,
Cell Signaling Technology
), supplemented with a complete protease and phosphatase
inhibitor
cocktail
(9)
Protocol for identifying physiologically relevant binding proteins of G-protein-coupled receptors
STAR Protocols
December 1, 2023
Bill X. Huang, Hee-Yong Kim
sc-362305
Precision plus protein standards,
Bio-Rad
,Cat#:
161-0374
Cell lysis buffer,Cell Signaling Technology,Cat#:
9803
PMSF
,
Cell Signaling Technology
,Cat#:
8553
BSA
,Millipore Sigma,Cat#: 126609 DAPI,Millipore Sigma,Cat#:
(10)
Simplified Synthesis of Renieramycin T Derivatives to Target Cancer Stem Cells via β-Catenin Proteasomal Degradation in Human Lung Cancer
Mar Drugs
November 30, 2023
Zin Zin Ei, Satapat Racha, Masashi Yokoya, Daiki Hotta, Hongbin Zou, Pithi Chanvorachote
NaCl, 5 mM EDTA, 1% Triton X-100, 1 mM phenylmethylsufonyl fluoride, and 2 μg/mL
pepstatin A
(cat no:
9803
,
Cell Signaling
), along with complete protease
inhibitor
cocktail tablets provided in EASYpack (Roche,
(11)
Proteomic analysis of exosomes secreted during the epithelial-mesenchymal transition and potential biomarkers of mesenchymal high-grade serous ovarian carcinoma
Journal of Ovarian Research
November 29, 2023
Germano Aguiar Ferreira, Carolina Hassibe Thomé, Clarice Izumi, Mariana Lopes Grassi, Guilherme Pauperio Lanfredi, Marcus Smolka, Vitor Marcel Faça, Francisco José Candido dos Reis
[6].
Protein extraction
The cells were washed with PBS twice and subsequently disrupted in lysis buffer (Cat#
9803
,
Cell Signaling
, Danvers, MA, USA) using three
sonication
cycles. Each
sonication
cycle lasted for 5
(12)
Spatial Distribution of Non-Immune Cells Expressing Glycoprotein A Repetitions Predominant in Human and Murine Metastatic Lymph Nodes
Cancers (Basel)
November 28, 2023
Loïc Rouaud, Louis Baudin, Marine Gautier-Isola, Pierre Van Meerbeeck, Emilie Feyereisen, Silvia Blacher, Nicolas van Baren, Frédéric Kridelka, Sophie Lucas, Agnes Noel
EGTA
, 1% Triton, 2.5 mM sodium pyrophosphate, 1 mM β-glycerophosphate, 1 mM Na3VO4, 1 µg/mL leupeptin (#
9803
,
Cell Signaling
, Danvers, MA, USA), and 1× protease/phosphatase
inhibitor
cocktail (Complete and
phosSTOP
,
(13)
Protective role of IL-17-producing γδ T cells in a laser-induced choroidal neovascularization mouse model
Journal of Neuroinflammation
November 25, 2023
Yu-Hsien Chang, Chung-Hsi Hsing, Chiao-Juno Chiu, Yi-Rou Wu, Sheng-Min Hsu, Yu-Hsiang Hsu
luminometer.
Western blotting
Mouse
ocular tissues or ARPE-19 cells were lysed with commercial Cell Lysis Buffer (#
9803
,
Cell Signaling
, Beverly, MA, USA). Proteins were separated by
SDS‒PAGE
and transferred electrophoretically
(14)
Regulated Induced Proximity Targeting Chimeras (RIPTACs): a Novel Heterobifunctional Small Molecule Therapeutic Strategy for Killing Cancer Cells Selectively
bioRxiv
November 22, 2023
Kanak Raina, Chris D. Forbes, Rebecca Stronk, Jonathan P. Rappi, Kyle J. Eastman, Samuel W. Gerritz, Xinheng Yu, Hao Li, Amit Bhardwaj, Mia Forgione,
et al.
instructions with some modifications. Briefly, HFL cell pellet was resuspended in 500μL of 1X lysis buffer (#
9803
,
Cell Signaling
) for 30min at 4°C, vortex every 10 min, and then the suspension was pelleted at 14000
(15)
Liver cancer stem cell dissemination and metastasis: uncovering the role of NRCAM in hepatocellular carcinoma
Journal of Experimental & Clinical Cancer Research
November 22, 2023
Lingyun Zhou, Linye He, Chang-Hai Liu, Huandi Qiu, Li Zheng, Klarke Michael Sample, Qin Wu, Jiaxin Li, Kunlin Xie, Javier Ampuero,
et al.
content of cells and tissues was extracted with Radio
Immunoprecipitation Assay
(RIPA) lysis buffer (#
9803
,
Cell Signaling Technology
, US) following the manufacturer’s instructions; 50 μg of total protein was
(16)
Modification of lysine-260 2-hydroxyisobutyrylation destabilizes ALDH1A1 expression to regulate bladder cancer progression
iScience
November 17, 2023
Zhilei Zhang, Yonghua Wang, Zhijuan Liang, Zhaoyuan Meng, Xiangyan Zhang, Guofeng Ma, Yuanbin Chen, Mingxin Zhang, Yinjie Su, Zhiqiang Li,
et al.
Western blotting The
total protein content was extracted by Cell Lysis Buffer (
Cell Signaling Technology
, #
9803
), and the proteins were analyzed by
Western blotting
following the standard methods, while the immune
(17)
KSR1 knockout mouse model demonstrates MAPK pathway’s key role in cisplatin- and noise-induced hearing loss
bioRxiv
November 17, 2023
Matthew A. Ingersoll, Richard D. Lutze, Regina G. Kelmann, Daniel F. Kresock, Jordan D. Marsh, Rene V. Quevedo, Jian Zuo, Tal Teitz
dissected on ice to isolate the organ of Corti, which was homogenized to prepare lysates in lysis buffer (
9803
;
Cell Signaling Technology
) after adding protease and phosphatase
inhibitors
(Roche). Each sample consisted
(18)
ERK1/2 Inhibition Alleviates Noise-Induced Hearing Loss While Tempering Down the Immune Response
bioRxiv
November 16, 2023
Richard D. Lutze, Matthew A. Ingersoll, Alena Thotam, Anjali Joseph, Joshua Fernandes, Tal Teitz
were sacrificed 6 days after noise exposure and whole cochlear lysates were prepared in lysis buffer (
9803
;
Cell Signaling
) after adding protease (cOmplete ULTRA Tablets 05892791001) and phosphatase (
PhosSTOP
(19)
Aryl hydrocarbon receptor regulates IL-22 receptor expression on thymic epithelial cell and accelerates thymus regeneration
npj Regenerative Medicine
November 8, 2023
Jingyi Shen, Ying Wang, Fei Zheng, Shuo Cao, Qiu Lan, Kailin Xu, Bin Pan
–ΔΔCT values.
Western blot
Proteins were extracted from cells or tissues using Cell Lysis Buffer (
9803
,
Cell Signaling Technology
, Danvers, MA, USA). The following antibodies were used.
STAT3
(
4904
) and
(20)
Gab2 promotes the growth of colorectal cancer by regulating the M2 polarization of tumor‑associated macrophages
International Journal of Molecular Medicine
November 8, 2023
Xuehan Gao, Runying Long, Ming Qin, Wenfang Zhu, Linna Wei, Pinzhi Dong, Jin Chen, Junmin Luo, Jihong Feng
analysis Proteins were extracted from the PMΦ, BMDM, THP-1, Tu-TAM, TCM-TAM using
cell lysis
buffer (cat. no.
9803
;
Cell Signaling Technology
, Inc.), followed by
centrifugation
at 12,000 × g for 10 min at 4°C. The protein
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Reviews
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(1)
10/30/2015
Cell Lysis Buffer That Works
PENG SHI
This cell lysis buffer is used to lyse cells under nondenaturing conditions. The lysis effect in relatively mild and we use it together with other reagents and could get satisfactory total protein ...
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Cell Lysis Buffer (10X) from Cell Signaling Technology
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