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Protein Biochemistry
Protein Purification
Lysis Buffers / Cell Lysis Kits
Cell Lysis Buffer (10X)
Cell Lysis Buffer (10X) from Cell Signaling Technology
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Citations:
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Supplier Page
Supplier Page from
Cell Signaling Technology for
Cell Lysis Buffer (10X)
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Company Info
Citations
Reviews
Product Specs
Item
Cell Lysis Buffer (10X)
Company
Cell Signaling Technology
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
9803S
Quantity
15 ml
Research Area
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Cell Signaling Technology
3 Trask Lane
Danvers
,
Massachusetts
01923
United States
Phone
: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488
Company Profile
Website:
http://www.cellsignal.com/
Website:
Technical Support
Citations (1233)
(81)
The CD40 agonist HERA-CD40L results in enhanced activation of antigen presenting cells, promoting an anti-tumor effect alone and in combination with radiotherapy
Frontiers in Immunology
May 26, 2023
Jamie Frankish, Debayan Mukherjee, Erminia Romano, Katharina Billian-Frey, Matthias Schröder, Karl Heinonen, Christian Merz, Mauricio Redondo Müller, Christian Gieffers, Oliver Hill,
et al.
Cells were lysed for 30 mins on ice without agitation with Cell Lysis Buffer (
Cell signaling
, Cat. No.
9803
) supplemented with
PMSF
(Cell Signaling Cat. No.
8553
),
Protease
Inhibitor
(cOmplete™, Mini, EDTA-free
(82)
Role of p53 in Cisplatin-Induced Myotube Atrophy
International journal of molecular sciences
May 24, 2023
Matsumoto C, Sekine H, Zhang N, Mogami S, Fujitsuka N, Takeda H.
Western Blotting
A
cell lysis
buffer containing a protease
inhibitor
and serine phosphatase
inhibitor
(10×) (#
9803
,
Cell Signaling Technology
, Danvers, MA, USA) was used to isolate proteins from C2C12
myotubes
(3.7
(83)
Multi-omics of Bohring-Opitz syndrome truncating ASXL1 mutations identify canonical and non-canonical Wnt signaling dysregulation
JCI Insight
May 22, 2023
Isabella Lin, Angela Wei, Zain Awamleh, Meghna Singh, Aileen Ning, Analeyla Herrera, REACH Biobank and Registry, Bianca E. Russell, Rosanna Weksberg, Valerie A. Arboleda
Western blotting
. Whole-cell lysates were extracted with Cell Lysis Buffer (10×,
Cell Signaling Technology
,
9803S
),
cytoplasm
and nuclei extracted using the CE/NER Kit (
Thermo Fisher Scientific
,
78833
), and histones
(84)
Loss of Astrocytic µ Opioid Receptors Exacerbates Aversion Associated with Morphine Withdrawal in Mice: Role of Mitochondrial Respiration
Cells
May 17, 2023
Kateryna Murlanova, Yan Jouroukhin, Ksenia Novototskaya-Vlasova, Shovgi Huseynov, Olga Pletnikova, Michael J. Morales, Yun Guan, Atsushi Kamiya, Dwight E. Bergles, David M. Dietz,
et al.
sorting, cells were centrifuged for 5 min at 300× g at 4 °C. Pellets were dissolved in
cell lysis
buffer (
9803
,
Cell Signaling Technology
, Danvers, MA, USA)
for Western blotting
or in AstroMACS medium (130-117-031,
(85)
Short-Term TERT Inhibition Impairs Cellular Proliferation via a Telomere Length-Independent Mechanism and Can Be Exploited as a Potential Anticancer Approach
Cancers
May 9, 2023
Amin A, Morello M, Petrara MR, Rizzo B, Argenton F, De Rossi A, Giunco S.
proteins. For
co-immunoprecipitation
, cells were lysed
in immunoprecipitation
(IP)
cell lysis
buffer (
9803
,
Cell Signaling
) on ice, followed by 10 min of
centrifugation
at 14,000 rpm. The proteins in the supernatants
(86)
25KDa branched polyethylenimine increases interferon-γ production in natural killer cells via improving translation efficiency
Cell Communication and Signaling
May 9, 2023
Eun-Su Ko, Seung Hee Choi, Minwook Lee, Kyung-Soon Park
listed in Additional file 1: Table S1.
Immunoblot
analysis Cells were lysed in
cell lysis
buffer (
9803S
,
Cell Signaling Technology
) supplemented with a protease and phosphatase
inhibitor
cocktail (
87786
,
(87)
Sexual dimorphism in bidirectional SR-mitochondria crosstalk in ventricular cardiomyocytes
Basic Research in Cardiology
May 3, 2023
Richard T. Clements, Radmila Terentyeva, Shanna Hamilton, Paul M. L. Janssen, Karim Roder, Benjamin Y. Martin, Fruzsina Perger, Timothy Schneider, Zuzana Nichtova, Anindhya S. Das,
et al.
proteins Tissue samples or cultured
rat
VCMs were lysed in lysis buffer from
Cell Signaling
(catalogue no.
9803S
), supplemented with phosphatase (Calbiochem, San Diego, CA, USA; catalogue no. 524625) and protease
(88)
FALCON systematically interrogates free fatty acid biology and identifies a novel mediator of lipotoxicity
Cell metabolism
May 2, 2023
Wieder N, Fried JC, Kim C, Sidhom EH, Brown MR, Marshall JL, Arevalo C, Dvela-Levitt M, Kost-Alimova M, Sieber J,
et al
.
iodide-negative cells per total number of cells per well (n=4).
Western Blot
MIN6 cells were lysed (#
9803
,
Cell Signaling Technology
) in the presence of
protease inhibitors
(#05892791001, Roche) and phosphatase
(89)
Stretch regulates alveologenesis and homeostasis via mesenchymal Gαq/11-mediated TGFβ2 activation
Development (Cambridge, England)
May 1, 2023
Goodwin AT, John AE, Joseph C, Habgood A, Tatler AL, Susztak K, Palmer M, Offermanns S, Henderson NC, Jenkins RG.
in identical conditions alongside the Flexcell® apparatus. Cells were lysed in protein lysis buffer (
9803
,
Cell Signaling Technology
) supplemented with phosphatase (Phos-Stop, 04906837001, Sigma-Aldrich) and
(90)
Uropathogenic Escherichia coli infection-induced epithelial trained immunity impacts urinary tract disease outcome
Nature microbiology
May 1, 2023
Russell SK, Harrison JK, Olson BS, Lee HJ, O'Brien VP, Xing X, Livny J, Yu L, Roberson EDO, Bomjan R,
et al
.
used for the casp1 heat map.
Immunoblotting
Cells were lysed with
cell lysis
buffer (
Cell Signaling
,
9803S
) according to the manufacturer’s instructions. Rapid Gold
BCA Protein Assay kit
was used to determine
(91)
Macroscopic quorum sensing sustains differentiating embryonic stem cells
Nature chemical biology
May 1, 2023
Daneshpour H, van den Bersselaar P, Chao CH, Fazzio TG, Youk H.
centrifuged them to form a pellet. We then lysed the cells with a lysis buffer (
Cell Signaling Technology
,
9803
) and 1 mM of
PMSF
(Sigma-Aldrich, P7626). We performed a sandwich-ELISA assay by incubating the cell
(92)
Notch1 and Notch4 core binding domain peptibodies exhibit distinct ligand-binding and anti-angiogenic properties
Angiogenesis
May 1, 2023
Timothy Sargis, Seock-Won Youn, Krishna Thakkar, L. A. Naiche, Na Yoon Paik, Kostandin V. Pajcini, Jan K. Kitajewski
30 min. The cells were subsequently lysed in 100 μl of 1 ×
cell lysis
buffer from
Cell Signaling
(#
9803
). The lysate was pulled down by 20 μl of Protein A/G magnetic beads (
Thermo Fisher Scientific
). To reverse
(93)
Single-cell transcriptome analyses reveal critical roles of RNA splicing during leukemia progression
PLoS biology
May 1, 2023
Wu B, Chen X, Pan X, Deng X, Li S, Wang Z, Wang J, Liao D, Xu J, Chen M,
et al
.
(
BD Biosciences
).
Western blot
Cells were lysed with
cell lysis
buffer (
Cell Signaling Technology
, Cat#
9803
), and cell lysate was clarified by
centrifugation
, according to the manufacturer’s protocol. Anti-
NPM1
(94)
Resveratrol Alleviates Diabetic Periodontitis-Induced Alveolar Osteocyte Ferroptosis Possibly via Regulation of SLC7A11/GPX4
Nutrients
April 28, 2023
Yue Li, Zhijun Huang, Shuaifei Pan, Yuhui Feng, Haokun He, Shuguang Cheng, Lijing Wang, Liping Wang, Janak Lal Pathak
Capillary-Based
Immunoassay
Whole-cell lysates were extracted using a total
protein extraction
kit (#
9803
,
CST
, Fall River, MA, USA). Protein separation and detection were performed using an automated
capillary electrophoresis
(95)
Soluble pathogenic tau enters brain vascular endothelial cells and drives cellular senescence and brain microvascular dysfunction in a mouse model of tauopathy
Nature communications
April 25, 2023
Hussong SA, Banh AQ, Van Skike CE, Dorigatti AO, Hernandez SF, Hart MJ, Ferran B, Makhlouf H, Gaczynska M, Osmulski PA,
et al
.
swing bucket rotor. The pellet was resuspended in 1X
cell lysis
buffer (
Cell Signaling Technology
,
9803
, Danvers, MA, USA) with
protease inhibitors
(cOmplete mini, Roche, Indianapolis, IN, USA), briefly sonicated,
(96)
Neuropilin-1 Knockout and Rescue Confirms Its Role to Promote Metastasis in MDA-MB-231 Breast Cancer Cells
International Journal of Molecular Sciences
April 25, 2023
Noura Al-Zeheimi, Yan Gao, Peter A. Greer, Sirin A. Adham
Briefly, the cells were washed with ice-cold PBS and incubated with lysis buffer for 1–2 min (Cat number
9803
,
Cell Signaling Technology
, Trask Lane, Danvers, MA 01923, USA) in the presence of 0.1 mM phenylmethylsulfonyl
(97)
Secreted Soluble Factors from Tumor-Activated Mesenchymal Stromal Cells Confer Platinum Chemoresistance to Ovarian Cancer Cells
International journal of molecular sciences
April 23, 2023
Koren Carmi Y, Khamaisi H, Adawi R, Noyman E, Gopas J, Mahajna J.
on an 8–12% acrylamide gel. Cell lysate samples were prepared for loading by adding lysis buffer (#
9803
Cell Signaling Technology
, Danvers, MA, USA), containing
protease inhibitors
(P8340 and
P5726
,
Sigma
,
(98)
Autophagy supports mitochondrial metabolism through the regulation of iron homeostasis in pancreatic cancer
Science advances
April 21, 2023
Mukhopadhyay S, Encarnación-Rosado J, Lin EY, Sohn ASW, Zhang H, Mancias JD, Kimmelman AC.
Western blot analysis
, lysates were prepared using the Cell Lysis Buffer (1×,
Cell Signaling Technology
:
9803
) supplemented with protease
inhibitor
(Thermo, A32953) and phosphatase
inhibitor
(PhosSTOP; Sigma-Aldrich,
(99)
Interaction between GPR110 (ADGRF1) and tight junction protein occludin implicated in blood-brain barrier permeability
iScience
April 21, 2023
Huang BX, Chen H, Joo Y, Kwon HS, Fu C, Spector AA, Kim HY.
sc-362305
Precision plus protein standards,
Bio-Rad
,Cat#:
161-0374
Cell lysis buffer,Cell Signaling Tech.,Cat#:
9803
PMSF
,
Cell Signaling
Tech.,Cat#:
8553
BSA
,Millipore Sigma,Cat#: 126609 Vitamin E,
Millipore Sigma
,Cat#:
(100)
Identification and characterization of the CDK1-BMAL1-UHRF1 pathway driving tumor progression
iScience
April 21, 2023
Dan Wang, Fenglin Wang, Shengfeng Wang, Ling Chu, Daolin Tang, Pan Chen, Minghua Yang
A2220
Monoclonal Anti-HA-Agarose,
Sigma
,Cat#
A2095
Cell Lysis Buffer,
Cell Signaling Technology
,Cat#
9803
BCA Protein Assay Kit
,
Thermo Scientific
,Cat#
23235
LDS Sample Buffer,Invitrogen,Cat# NP007 PageRuler
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Reviews
Write a Review
(1)
10/30/2015
Cell Lysis Buffer That Works
PENG SHI
This cell lysis buffer is used to lyse cells under nondenaturing conditions. The lysis effect in relatively mild and we use it together with other reagents and could get satisfactory total protein ...
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Cell Lysis Buffer (10X) from Cell Signaling Technology
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