Adapted to Express Five® SFM. (Cat. No. 10486-025) High Five™ Cells (BTI-TN-5B1-4) are a clonal isolate, derived from the parental Trichopulsia ni
cell line and are commonly used for the expression of recombinant proteins using the Baculovirus Expression Vector System (BEVS). The cells are adapted to serum-free suspension culture in Express Five® SFM which saves significant time and expense associated with the adaptation of cultures to serum-free and/or suspension cultures. Express Five® SFM is a protein-free insect cell culture medium optimized to support maximum cell growth and recombinant gene expression.
- Provided as a frozen stock in Express Five¤ SFM containing 7.5% DMSO.
- 3 x 106 cells/ml provided.
- Protocols for recovery, culture, and cryopreservation are provided on the product insert.
Applications: For baculovirus propagation and recombinant protein expression.Performance testing: Each lot is tested for growth and viability post recovery from cryopreservation and is also tested for the absence of mycoplasma.