FuGENE 6 Transfection Reagent from Promega

Product Specs
ItemFuGENE 6 Transfection Reagent
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE2693
Quantity0.5ml
Molecular Weight~71kDa
Transfection MethodNon-liposomal
Transfection Sample TypeDNA, and Plasmid

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Nicotinamide N-methyltransferase mediates lipofibroblast-myofibroblast transition and apoptosis resistanceThe Journal of biological chemistryAugust 1, 2023Rehan M, Deskin B, Kurundkar AR, Yadav S, Matsunaga Y, Manges J, Smith N, Dsouza KG, Burow ME, Thannickal VJ.plasmid were transfected per well of six well plate using FuGENE6 Transfection Reagent (Catalogue Number:E2693) from Promega Corporation, as per manufacturer’s instructions. For NNMT overexpression, control (pCMV-3-C-HA-NCV,

(2) Mutational screens highlight glycosylation as a modulator of colony-stimulating factor 3 receptor (CSF3R) activityJournal of Biological ChemistryJune 1, 2023Michael J. Hollander, Stacy A. Malaker, Nicholas M. Riley, Idalia Perez, Nayla M. Abney, Melissa A. Gray, Julia E. Maxson, Jennifer R. Cochran, Carolyn R. Bertozzi(Thermo Fisher Scientific 31985062) was mixed with 10 μl of FuGENE 6 transfection reagent (Promega E2693). After 5 min, 2 μg of receptor plasmid (1 μg each for coreceptors) and 0.83 μg of the EcoPac packaging

(3) Protocol for quantitative evaluation of the impact of paracrine senescence on cellular reprogramming in cultured cells and mouse modelsSTAR ProtocolsMarch 1, 2023Jérémy Chantrel, Cheng Chen, Jun Zhang, Han Liinhibitory factor (LIF),Miltenyi Biotec,130-095 Doxycycline (DOX),Sigma-Aldrich,9891 Fugene6,Promega,E2693 Polybrene (0.8 mg/mL stock solution),ABP Biosciences,D025 Cardiotoxin,Merck Chimie SAS,217503 2-methylbutane,Sigma-Aldrich,320404-1L Tragacanth,VWR

(4) [1,2,4]Triazolo[3,4-b]benzothiazole Scaffold as Versatile Nicotinamide Mimic Allowing Nanomolar Inhibition of Different PARP EnzymesJournal of Medicinal ChemistryJanuary 26, 2023Sudarshan Murthy, Maria Giulia Nizi, Mirko M. Maksimainen, Serena Massari, Juho Alaviuhkola, Barbara E. Lippok, Chiara Vagaggini, Sven T. Sowa, Albert Galera-Prat, Yashwanth Ashok, et al.was produced as described before.25 Sf21 cells were transfected with bacmids using Fugene6 (Promega #E2693). V0 virus containing media was harvested after 7 days. These viruses were amplified to increase titre

(5) CD44v3,8‐10 is essential for Slug‐dependent vimentin gene expression to acquire TGF‐β1‐induced tumor cell motilityCancer ScienceAugust 1, 2022Shichao Qiu, Makoto Iimori, Keitaro Edahiro, Yoshiaki Fujimoto, Kazuaki Matsuoka, Eiji Oki, Yoshihiko Maehara, Masaki Mori, Hiroyuki Kitaotransfected with 500 ng of each reporter plasmid in 24‐well plates using FuGENE6 Transfection Reagent (E2693; Promega). Luciferase activity was measured using the Dual‐Luciferase Reporter Assay System (E1910;

(6) Targeted long-read sequencing identifies missing pathogenic variants in unsolved Werner syndrome casesJournal of Medical GeneticsMay 9, 2022Danny E. Miller, Lin Lee, Miranda Galey, Renuka Kandhaya-Pillai, Marc Tischkowitz, Deepak Amalnath, Avadh Vithlani, Koutaro Yokote, Hisaya Kato, Yoshiro Maezawa, et al.constructs were transfected to immortalised control human fibroblasts, 82-6hTERT, using FuGENE 6 (catalogue# E2693, Promega). After 48 hours, total RNA was isolated and reverse-transcribed with High-Capacity cDNA Reverse

(7) Paired heavy- and light-chain signatures contribute to potent SARS-CoV-2 neutralization in public antibody responsesCell ReportsOctober 1, 2021Bailey B. Banach et al.Monarch DNA Gel Extraction Kit,NEB,Cat #T1020 NEBuilder HiFi DNA,NEB,Cat #E5520 SDCAA medium,Teknova,Cat#2S0540 FuGENE 6,Promega,Cat #E2693 ACE2-Fc,Neil King and David Veesler at the University of Washington.,N/A NHS-Ester biotin,ThermoFisher

(8) STING nuclear partners contribute to innate immune signaling responsesiScienceSeptember 24, 2021Charles R. Dixon, Poonam Malik, Jose I. de las Heras, Natalia Saiz-Ros, Flavia de Lima Alves, Mark Tingey, Eleanor Gaunt, A. Christine Richardson, David A. Kelly, Martin W. Goldberg, et al.T7575 jetPrime transfection reagent,Polyplus,Cat# 114-15 Fugene 6 transfection reagent,Promega,Cat# E2693 Superscript II reverse transcriptase,Invitrogen,Cat# 100004925 ,, Critical commercial assays,Critical

(9) Protocol for the generation of pancreatic and hepatic progenitors from human pluripotent stem cells for gene regulatory assaysSTAR ProtocolsJune 1, 2021Lay Shuen Tan, Resilind Su Ern Chew, Natasha Hui Jin Ng, Adrian Kee Keong TeoDual-Luciferase® Reporter 1000 Assay System,Promega,Cat # E1980 FuGENE® 6 Transfection Reagent,Promega,"Cat # E2691, E2692 and E2693" ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, CF-1

(10) Restoration of SIRT3 gene expression by airway delivery resolves age-associated persistent lung fibrosis in miceNat AgingFebruary 1, 2021Rehan M, Kurundkar D, Kurundkar AR, Logsdon NJ, Smith SR, Chanda D, Bernard K, Sanders YY, Deshane JS, Dsouza KG, et al.plasmid were transfected per well of 6 well plate using FuGENE®6 Transfection Reagent (Catalogue Number:E2693 ) from Promega Corporation, as per manufacturer’s instructions. Macrophages were transfected using Effectene® transfection reagent

(11) Targeting PAK4 to reprogram the vascular microenvironment and improve CAR-T immunotherapy for glioblastomaNature cancerJanuary 1, 2021Ma W, Wang Y, Zhang R, Yang F, Zhang D, Huang M, Zhang L, Dorsey JF, Binder ZA, O'Rourke DM, et al.pMEF2-fLuc reporter vector (Signosis; LR-2055) and the Renilla-Luc vector using FuGene 6 reagent (Promega; E2693) in serum-free Opti-MEM medium for 6 h, followed by recovery with serum-supplemented medium for 24 h.

(12) A heterodimeric SNX4--SNX7 SNX-BAR autophagy complex coordinates ATG9A trafficking for efficient autophagosome assemblyJournal of cell scienceJuly 15, 2020Antón Z, Betin VMS, Simonetti B, Traer CJ, Attar N, Cullen PJ, Lane JD.co-transfected with CRISPR-Cas9 plasmids and a plasmid conferring puromycin resistance using FuGENE (Promega, E2693). 24 h after transfection, cells were subjected to 24 h of puromycin selection (Sigma, P8833; 2 µg/ml),

(13) Generation of a Tumor-Specific Chemokine Gradient Using Oncolytic Vesicular Stomatitis Virus Encoding CXCL9Molecular therapy oncolyticsMarch 27, 2020Eckert EC, Nace RA, Tonne JM, Evgin L, Vile RG, Russell SJ.μg of VSV plasmid, 0.5 μg of pN, 0.4 μg of pP, and 0.2 μg of pL all in the pCI vector with FuGENE 6 (E2693; Promega), according to the manufacturer’s instructions.45 The transfection complexes were added dropwise

(14) USP7-Dependent Regulation of TRAF Activation and Signaling by a Viral Interferon Regulatory Factor HomologueJournal of VirologyJanuary 6, 2020Qiwang Xiang, Hyunwoo Ju, John Nicholasvirus production, BAC16 DNA was transfected into iSLK cells with FuGENE 6 transfection reagent (catalogue number E2693; Promega), and BAC16-containing cells (green fluorescent protein [GFP] positive) were selected by treatment

(15) LDL receptor related protein 1 requires the I3 domain of discs-large homolog 1/DLG1 for interaction with the kinesin motor protein KIF13BBiochimica et Biophysica Acta (BBA) - Molecular Cell ResearchDecember 1, 2019Joslyn Mills, Toshihiko Hanada, Yoichi Hase, Laura Liscum, Athar H. Chishtiwere purchased from Gibco (Thermo Fisher Scientific). FuGENE 6 for transfections was from Promega (E2693). LRP1-Herz was obtained from the laboratory of Dr. Joachim Herz [30], [3H] Cholesteryl oleate (NET746)

(16) No evidence for viral small RNA production and antiviral function of Argonaute 2 in human cellsScientific reportsSeptember 24, 2019Schuster S, Overheul GJ, Bauer L, van Kuppeveld FJM, van Rij RP.the cells were transfected with 200 ng or 2 μg of poly (I:C) (GE Healthcare) using FuGENE 6 (Promega E2693). RNA was isolated after an incubation for 6 h. HEK293T and BHK-21 cells were cultured in Dulbecco’s

(17) Mitochondria supply ATP to the ER through a mechanism antagonized by cytosolic Ca2+eLifeSeptember 9, 2019Jing Yong, Helmut Bischof, Sandra Burgstaller, Marina Siirin, Anne Murphy, Roland Malli, Randal J Kaufman"Promega Corporation, Madison, USA",# E2431, Commercial assay or kit,FuGENE 6 Transfection Reagent,Promega,# E2693, Commercial assay or kit,Calcium Assay Kit,Adipogen Corp.,# JAI-CCA-030, Commercial assay or kit,XF24

(18) Construction of Viral Vectors for Cell Type-specific CRISPR Gene Editing in the Adult Mouse BrainBIO-PROTOCOLAugust 20, 2019Hiroshi Yamaguchi, Luis de LeceaProcedure C) 28. PrimeSTAR HS DNA polymerase 29. FuGENE6 Transfection Reagent (Promega, catalog number: E2693) 30. DMEM (1x) (Gibco, catalog number: 11995-065) 31. Fetal Bovine Serum (Corning, catalog number: 35-015-CV) 32.

(19) Absolute quantification of cohesin, CTCF and their regulators in human cellseLifeJune 17, 2019Johann Holzmann et al.plasmid #54759, kindly provided by J Lippincott-Schwartz) using FuGENE6 Transfection Reagent (Promega; #E2693) according to the manufacturer’s instructions, while HK WT cells in another well remained untransfected.

(20) Determining cellular CTCF and cohesin abundances to constrain 3D genome modelseLifeJune 17, 2019Cattoglio C, Pustova I, Walther N, Ho JJ, Hantsche-Grininger M, Inouye CJ, Hossain MJ, Dailey GM, Ellenberg J, Darzacq X, et al.transiently transfected with 150 ng pHTCHaloTag plasmid using FuGENE6 Transfection Reagent (Promega; #E2693) according to the manufacturer’s instructions. On the day of imaging, cells were labeled with 500 nM

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(1)

2/4/2020Best Transfection Reagent for Large-Sized DNA Sunita MeenaGood for large-sized DNA.
(1)

6/15/2016Fugene 6 Transfection Kit Benjamin GerovacThis kit is used to transfect mammalian cell lines. The kit is simple to use and results in high transfection efficiency.