Best Transfection Reagent for Large-Sized DNA

National Dairy Research Institute
Animal Biochemistry
Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Promega

Product Name:

FuGENE® 6 Transfection Reagent

Catalog Number:

E2693

Cells that were subjected to transfection were incubated in Opti-MEM media containing 6.8kb plasmid DNA packed in FuGENE® 6 transfection reagent. After transfection, cells were kept back in complete media for at least 36 hours and analyzed.

Experimental Design and Results Summary

Application

Cell transfection

Starting Material

Cells and Plasmid DNA

Tips

None

Results Summary

The transfection efficiency with FuGENE® 6 transfection reagent was found to be >90 per cent. Moreover, this transfection reagent has shown less cytotoxicity due to membrane damage.

DOI or PMID #

None

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Summary

The Good

Has best transfection efficiency for larger sized DNA

The Bad

None

The Bottom Line

Good for large-sized DNA

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