Reversed Phase

Reverse phase chromatography is a specific type of liquid chromatography that has a non-polar, or hydrophobic, stationary phase through which a polar, or hydrophilic, mobile phase is eluted. Nucleic acid analysis, separation and isolation of biomolecules, and therapeutic drug monitoring can be done using reverse phase chromatography. As the mobile phase moves through the column, the different components adsorb to it with varying affinities. They are separated and detected as they move out of the stationary phase. One of the reasons reverse phase chromatography is so useful is because the mobile phase can be made more or less hydrophilic, the pH can be changed, and the temperature can be altered to provide the optimum conditions for a wide range of samples to be analyzed.

Reversed Phase

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