The Biocompare Cells and Microorganisms search tool is a diverse catalog of primary cells, cell lines, and microbial strains from many suppliers. Cell lines include stable immortalized lines, cells overexpressing target genes, hybridomas, reporter cell lines, and knockout cells. It features cells from various lineages, including myeloid and lymphoid cells, neuronal cells, fibroblasts, endothelial cells, hepatocytes, myocytes, and more. Users can also search for specific cells based on tissue type or host species, providing flexibility for a wide array of research applications. Important considerations when choosing cells for research include cell line authentication, culturing requirements, genetic or phenotypic modifications, and the intended downstream application. Inspect the product datasheets from the cell supplier pages for more detailed information.
Peripheral blood mononuclear cells, or PBMC, are cells possessing a single, round nucleus and are collected from the peripheral, or circulating, blood. It is typically isolated from whole blood, by density centrifugation with Ficoll, a polysaccharide. They are then collected in a middle layer called the buffy coat, which comprise about 1% of the given sample and are mostly depleted of red blood cells and platelets. PBMC are a rich source of lymphocytes, such as T cells, B cells, NK cells, and monocytes, such as macrophages, dendritic cells. From this population, further cell isolations are carried out by selecting for key protein surface markers such as CD4+ (T helper cells), CD8+ (cytotoxic T cells), CD14+ (monocytes), among others. PBMC are now readily available from a number of commercial sources, with samples isolated from both healthy and diseased individuals, and can be shipped fresh or frozen.