Anti-TOPBP1 antibodies are used in the immunodetection of the protein DNA topoisomerase II binding protein 1. In humans, the canonical protein has a reported length of 1522 amino acid residues and a mass of 170.7 kDa. Its subcellular localization is in the nucleus and cytoplasm. It is reported to be highly expressed in the heart, brain, placenta, lung and kidney. TOPBP1 is known to be required for DNA replication. Post-translational modifications have been described, including ubiquitination and phosphorylation. Synonyms for this target antigen include TOP2BP1, DNA topoisomerase 2-binding protein 1, DNA topoisomerase II-beta-binding protein 1, topoisomerase (DNA) II binding protein 1, and Dpb11. TOPBP1 gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Over 80 citations in the literature describe the use of TopBP1 antibodies in research. Western Blot is a widely used application for these antibodies. In addition, Immunohistochemistry is also a common application.