Anti-HSPB1 antibodies are used in the immunodetection of the protein heat shock protein family B (small) member 1. In humans, the canonical protein has a reported length of 205 amino acid residues and a mass of 22.8 kDa. Its subcellular localization is in the nucleus and cytoplasm. It is notably widely expressed in many tissue types. A member of the Small heat shock protein (HSP20) protein family, HSPB1 is known to be involved with intracellular signal transduction and the regulation of apoptosis. Post-translational modifications have been described, including phosphorylation. Synonyms for this target antigen include HEL-S-102, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27, and CMT2F. HSPB1 gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Over 850 citations in the literature describe the use of HSPB1 antibodies in research. Western Blot is a widely used application for these antibodies. Immunocytochemistry, Immunofluorescence, and Immunohistochemistry are also common applications.