Anti-podocalyxin like antibodies are used for the immunodetection of the protein encoded by the PODXL gene. In humans, the canonical protein has a reported length of 558 amino acid residues and a mass of 58.6 kDa. Its subcellular localization is in the cell membrane. Alternative splicing is reported to yield 2 different isoforms for this protein. A member of the Podocalyxin protein family, it is reported to be involved in regulation of both adhesion and cell morphology and cancer progression. Post-translational modifications have been described, including glycosylation.
The podocalyxin like marker can be used to characterize Podocytes.* Other names for this target antigen include PC, PCLP, PCLP-1, PDX, PODXL1, gp135, podocalyxin, and Gp200. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish and chimpanzee species. Over 640 citations in the literature describe the use of podocalyxin like antibodies in research. Flow Cytometry, Western Blot, Immunofluorescence, and Immunohistochemistry are common applications for these antibodies.
*HuBMAP Human Reference Atlas v1.4