Anti-CDC25B antibodies are used in the immunodetection of the protein cell division cycle 25B. In humans, the canonical protein has a reported length of 580 amino acid residues and a mass of 65 kDa. Its subcellular localization is in the cytoplasm. Up to 4 different isoforms have been reported for this protein. It is notably expressed in many tissues, such as the cerebral cortex, tonsil, and breast. A member of the MPI phosphatase protein family, CDC25B is known to be involved with the cell cycle. Post-translational modifications have been described, including phosphorylation. Synonyms for this target antigen include CDC25 homolog B, dual specificity phosphatase Cdc25B, and M-phase inducer phosphatase 2. CDC25B gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish and chimpanzee species. A number of CDC25B antibodies have been mentioned in research publications and have associated citations. Western Blot is a widely used application for these antibodies. ELISA, Immunofluorescence, and Immunohistochemistry are also common applications.