What are CytoSections?
CytoSections are FFPE sections of transiently transfected cDNA-specific gene targets expressed in human HEK293T cells, providing dependable and renewable positive control solutions to the IHC, ICC, and ISH users.
When can CytoSections be used?
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Positive Control for IHC/ICC/ISH Workflow:
Eliminate positive control hunting - CytoSections integrate into your existing workflow (click here to learn more)
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Validate Antibodies Faster:
Screen large pools for specific antibodies, including for those against rare mutations, without wasting tissues, time, or money (click here to learn more)
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Multicenter Reference:
Ideal reference for consistent results across labs
Key Features and Benefits
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Study Rare Gene Mutations:
- Access hard-to-find gene mutations with custom CytoSections
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Save Precious Tissue, Time, and Money
- Start experiments right away knowing that target expression for each lot is verified
- FFPE sections are comparable to your samples and easily incorporated into existing workflows
- Do not waste precious tissue for assay optimization and costly method development
- Once your assay is developed, cost effectively obtain as many slides as you need
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Increase Your Confidence in Generating Reproducible Date
- Transient transfection ensures consistent gene expression, unlike stable cell lines where expression can change over time, creating lot to lot variability
- No section-to-section expression variability
How are CytoSections made for your specific gene target?
TrueORF cDNA clones containing the gene of interest (GOI) are transiently transfected into mammalian cells. The transfected cells are spun down into a cell pellet, which is tested to validate the expression of the GOI before creating the FFPE block. Subsequently, the block is cut into thin slices and placed on a slide.
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