Anti-DTL antibodies are used in the immunodetection of the protein denticleless E3 ubiquitin protein ligase homolog. In humans, the canonical protein has a reported length of 730 amino acid residues and a mass of 79.5 kDa. Its subcellular localization is in the membrane, nucleus, and cytoplasm. Up to 2 different isoforms have been reported for this protein. It is reported to be expressed in the placenta and testis, very low expression seen in skeletal muscle. A member of the WD repeat cdt2 protein family, DTL is reported to be a substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. Post-translational modifications have been described, including ubiquitination and phosphorylation.
The DTL marker can be used to identify Cap1 General Capillary (cGap) Cells.* Synonyms for this target antigen include DCAF2, L2DTL, RAMP, denticleless protein homolog, DDB1- and CUL4-associated factor 2, and CDT2. DTL gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species.
*HuBMAP Human Reference Atlas v1.4