Anti-TIGIT antibodies are used in the immunodetection of the protein T cell immunoreceptor with Ig and ITIM domains. In humans, the canonical protein has a reported length of 244 amino acid residues and a mass of 26.3 kDa. Its subcellular localization is in the cell membrane. Up to 2 different isoforms have been reported for this protein. It is reported to be expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells. TIGIT is known to bind with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
The TIGIT marker can be used to identify Regulatory T Cells and Naive Regulatory T Cells.* Synonyms for this target antigen include V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain containing 3, V-set and transmembrane domain-containing protein 3, and V-set and immunoglobulin domain containing 9. Over 420 citations in the literature describe the use of TIGIT antibodies in research. Flow Cytometry is a widely used application for these antibodies. In addition, ELISA is also a common application.
*HuBMAP Human Reference Atlas v1.4