Fig 2: TIGIT is elevated on CD8+T cell in patients with GC and significantly reduced after D2 gastrectomy. (a) Flow cytometry analysis of CD8+ TIGIT+, CD8+PD-1+, or CD8+ TIGIT+PD-1+ T-cell populations in pre- and post-operative PBMC from patients with GC. (b) Frequency and number of CD8+ TIGIT+, CD8+PD-1+, or CD8+ TIGIT+PD-1+ cells in pre-chemotherapy and post-chemotherapy treated PBMC from patients with GC. **P < 0.01.

Fig 3: TIGIT and PD-1 expression in GC patients (a) Immunohistochemistry analysis of TIGIT, PD-1, and CD8 expression in gastric cancer (GC) tissues and adjacent normal tissues. (b) Kaplan Meier survival curve showed the association between TIGIT expression and over survival time in patients with GC (P < 0.0001). The patient number of high expression of TIGIT is 343 and the number of low expression is 98. (c) Kaplan Meier survival curve showed association between PD-1 expression and overall survival (OS) time in patients (P < 0.0001) The patient number of high expression of PD-1 is 310 and the number of low expression is 131.

Fig 4: Combination of SOX and anti-TIGIT is beneficial to CD8+ T cells proliferation and IFN-? release. (a, b) CD8+ TIL cells were treated with SOX, Anti-TIGIT, or SOX +Anti-TIGIT. Immunofluorescence was used to detect expression levels of KI-67. (c, d) CD8+ TIL cells were treated with SOX, Anti-TIGIT, or SOX +Anti-TIGIT, and stimulated with PMA for 5 h. Frequency and number of IFN-? of CD8+ cells were quantified. *P < 0.05, **P < 0.01.

Fig 5: The asscociation between TIGIT expression and GC patients outcome predicted online. (a) GEPIA database prediction of TIGIT expression in different types of tumour tissues and adjacent normal tissues. (b) Expression of TIGIT in gastric cancer (GC) tissues and adjacent normal tissues in GEPIA database. (c) Association between TIGIT expression and stage in GEPIA database. (d) Follow-up was available for the TIGIT expression and overall survival (OS) in Kaplan-Meier Plotter database (P = 9.6e-10). (e) Follow-up was available for TIGIT expression and FP in Kaplan-Meier Plotter database (P = 9.5e-09). (f) Follow-up was available for the TIGIT expression and PPS in Kaplan-Meier Plotter database (P = 1.8e-13). *P < 0.05.