Excellent Staining of TIGIT in PBMCs Surface

April 19, 2023

Infectious Diseases
Vall d'Hebron Institute of Research
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

anti-TIGIT AlexaFluor 647

Catalog Number:

51-9500-42

Clone Number:

MBSA43

Info:Supplier Page View Product Specs

Browse Similar Products: TIGIT Antibodies

This antibody offers excellent staining of the immune checkpoint receptor TIGIT on the surface of human PBMCs.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMCs obtaining with Ficoll density gradient of human blood cells

Primary Incubation

Cells were incubated for 20 min at RT with 0.63 ul of the antibody in 100 ul of Staining Buffer (PBS 1x 3% FBS)

Blocking Agent

Staining Buffer (PBS 1x 3% FBS)

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR Fortessa

Results Summary

This antibody offers excellent staining of the immune checkpoint receptor TIGIT on the surface of human PBMCs.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Excellent resolution with low amount of the antibody.

The Bad

Anything

The Bottom Line

I would buy it again.

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