Anti-MTHFD1 antibodies are used in the immunodetection of the protein methylenetetrahydrofolate dehydrogenase, cyclohydrolase and formyltetrahydrofolate synthetase 1. In humans, the canonical protein has a reported length of 935 amino acid residues and a mass of 101.5 kDa. Its subcellular localization is in the cytoplasm. It is ubiquitously expressed across many tissue types. A member of the Tetrahydrofolate dehydrogenase/cyclohydrolase protein family; Formate--tetrahydrofolate ligase protein family, MTHFD1 is reported to be a trifunctional enzyme that catalyzes the interconversion of three forms of one-carbon-substituted tetrahydrofolate: (6R)-5,10-methylene-5,6,7,8-tetrahydrofolate, 5,10-methenyltetrahydrofolate and (6S)-10-formyltetrahydrofolate. Synonyms for this target antigen include MTHFC, MTHFD, C-1-tetrahydrofolate synthase, cytoplasmic, 5,10-methylenetetrahydrofolate dehydrogenase, 5,10-methylenetetrahydrofolate cyclohydrolase, 10-formyltetrahydrofolate synthetase, and CIMAH. A number of MTHFD1 antibodies have been mentioned in research publications and have associated citations. Western Blot is a widely used application for these antibodies. Immunocytochemistry, Immunofluorescence, and Immunohistochemistry are also common applications.