Anti-CYP7A1 antibodies are used in the immunodetection of the protein cytochrome P450 family 7 subfamily A member 1. In humans, the canonical protein has a reported length of 504 amino acid residues and a mass of 57.7 kDa. Its subcellular localization is in the ER. It is notably expressed in the liver. A member of the Cytochrome P450 protein family, CYP7A1 is a cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols). Synonyms for this target antigen include CYP7, CYPVII, cytochrome P450 7A1, 24-hydroxycholesterol 7-alpha-hydroxylase, cholesterol 7-alpha-hydroxylase, and CP7A. CYP7A1 gene orthologs have been reported in the mouse, rat, bovine, frog, chimpanzee and chicken species. Over 50 citations in the literature describe the use of CYP7A1 antibodies in research. Western Blot is a widely used application for these antibodies. In addition, Immunofluorescence is also a common application.