Anti-ENOPH1 antibodies are used in the immunodetection of the protein enolase-phosphatase 1. In humans, the canonical protein has a reported length of 261 amino acid residues and a mass of 28.9 kDa. Its subcellular localization is in the nucleus and cytoplasm. Up to 2 different isoforms have been reported for this protein. It is notably expressed in many tissues, such as the appendix, thyroid gland, and adrenal gland. A member of the MasA/MtnC protein family, ENOPH1 is reported to be a bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene). Synonyms for this target antigen include 2,3-diketo-5-methylthio-1-phosphopentane phosphatase, acireductone synthase, and enolase-phosphatase E1. Some ENOPH1 antibodies may have been used in research and have associated citations. These antibodies are most commonly used in Western Blot experiments, among other applications.