Spheroids are 3-dimensional cell aggregates with important applications in cell-based experiments. Because spheroids mimic the microenvironment of different tissue types, they are a great cellular model, especially when investigating disease states and discovering new therapies. To simplify experimental workflows, speed discovery, and minimize variability, consider the mass production of spheroids.
With large scale production, researchers can quickly obtain uniform 3D cell material for many biomedical applications, such as DNA, RNA and protein analysis, cancer therapy screening, pluripotent cell differentiation, vesicle production, and 3D bioprinting.
Spheroids can be grown using ultra-low attachment surfaces. A hydrophilic, neutrally-charged coating keeps the cells in a suspended, unattached state that promotes spheroid formation in a scaffold-free model.
Taking it a step further, by introducing microcavity chambers within each microplate well, the capacity and density of growing spheroids can be drastically improved.
This microcavity technology is a defining feature of the Elplasia plates by Corning, which also utilizes the Ultra-Low Attachment surface. Elplasia Plates grant the ability to mass produce hundreds to thousands of spheroids of uniform size.
Elplasia vessels come in models that accommodate special culturing needs. The Elplasia 12K flask offers a convenient format that is similar to the standard T-75 flask. Using the microcavity geometry, it can grow roughly 12,000 spheroids at a time. This flask design is ideal for culturing large volumes for easy spheroid production and collection. The traditional vent cap/flask design helps to keep contamination at bay.
The Elplasia Open Well Plate comes in the standard microplate format, which makes it ideal for easy sampling and imaging of spheroids. As a microplate, it is also easily incorporated into automated processes. The Open Well Plate also accommodates useful features for media exchange, such as media ports.
Mass production of spheroids is a straightforward process. Prepare a flask or plate for seeding by rinsing with alcohol wetting solution. Add the single cell suspension. Culture the cells. To perform media exchanges, gently tilt the vessel and aspirate the supernatant. Take care not to disturb the spheroids. Finally, collect the spheroids by gentle shaking. Then, tilt the liquid out of cavities and wash.
Here are some additional considerations to help produce the best results.
It is important to start your culture using single cell suspensions. High quality suspensions with minimal cell debris and aggregation can help ensure the viability and uniformity of your spheroid culture. Optimize your seeding density based on the cell type, intended application, and duration of the culture. Work gently when handling vessels and changing the media, so that the growing spheroids are not disturbed.
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