Anti-DNMT3B antibodies are immunoglobulins that specifically recognize and bind DNMT3B, the gene encoding DNA (cytosine-5)-methyltransferase 3B in humans. In research applications, these antibodies are used to detect DNMT3B in tissue samples, cell lysates, and cultured cells. DNMT3B establishes de novo DNA methylation patterns during development, working alongside histone methylation to coordinate epigenetic gene silencing. Because de novo methylation shapes gene expression programs in development and is frequently disrupted in cancer, DNMT3B is a widely studied target in epigenetics research.
Catalog highlights and purchasing considerations
About the Biocompare DNMT3B antibody catalog
The Biocompare antibody search tool lists DNMT3B antibodies from a wide range of suppliers, with western blot, flow cytometry, and immunofluorescence among the most common validated applications. Several DNMT3B antibodies mentioned here have been cited across a range of epigenetics research, including studies on DNMT3B's nuclear interaction with methionine synthase and methionine adenosyltransferase, hypoxia-induced DNMT3B signaling in hepatocellular carcinoma, and DNMT3B1's role in developmentally regulated CpG island methylation.
Considerations when choosing a DNMT3B antibody for research
Confirm that the antibody's intended target is accurate, as many genes carry synonym symbols that can create ambiguity across suppliers. Specificity should be verified against the immunogen sequence or region listed on the product datasheet, since antibodies raised against different epitopes of the same protein can behave differently across applications. Beyond specificity, several target-related attributes can affect antibody binding, including subcellular location, extracellular and cytoplasmic domain boundaries for membrane-associated targets, tissue specificity, post-translational modifications, and the presence of additional isoforms or disease-associated variants. In parallel, antibody-specific selection criteria warrant consideration, including clonality (monoclonal versus polyclonal), host species, species reactivity, and conjugate or label choice suited to the detection method. Supplier validation data, such as western blot bands at the expected molecular weight, immunohistochemistry staining patterns consistent with known tissue distribution, and knockout or knockdown controls, should also be carefully reviewed before purchase.