Anti-Myosin VB antibodies are used for the immunodetection of the protein encoded by the MYO5B gene. In humans, the canonical protein has a reported length of 1848 amino acid residues and a mass of 213.7 kDa. Its subcellular localization is in the cytoplasm. Alternative splicing is reported to yield 3 different isoforms for this protein. A member of the Myosin protein family, it is suspected to be involved in vesicular trafficking via its association with the CART complex.
The myosin VB marker can be used to characterize Gray Matter MGE Interneurons and Gray Matter Splatter Neurons.* Other names for this target antigen include MVID1, PFIC10, unconventional myosin-Vb, MYO5B variant protein, and DIAR2. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish and chimpanzee species. A number of Myosin VB antibodies have been mentioned in research publications and have associated citations. Western Blot is a widely used application for these antibodies. In addition, ELISA is also a common application.
*HuBMAP Human Reference Atlas v1.4