Anti-SPN antibodies are used in the immunodetection of the protein sialophorin. In humans, the canonical protein has a reported length of 400 amino acid residues and a mass of 40.3 kDa. Its subcellular localization is in the membrane. It is notably expressed in the skin, lymph node, lung, bone marrow, and appendix. SPN is known to be involved with receptor-mediated signaling pathways and chemotaxis. Post-translational modifications have been described, including glycosylation, sumoylation, protein cleavage and phosphorylation.
The SPN marker can be used to identify Neutrophils.* Synonyms for this target antigen include GALGP, GPL115, LEU-22, LSN, leukosialin, galactoglycoprotein, leukocyte sialoglycoprotein, and CD43. SPN gene orthologs have been reported in the mouse, rat, bovine, frog and chimpanzee species. Over 630 citations in the literature describe the use of Spn antibodies in research. Flow Cytometry is a widely used application for these antibodies. Western Blot and Immunohistochemistry are also common applications.
*HuBMAP Human Reference Atlas v1.4