Fig 1: Expression patterns of CD31 and CD34 in E10.5 wild-type and SCL deletion embryos. Transverse sections of E10.5 wild-type (A) and SCL+/? (B), and SCL?/? (C) embryo proper were co-stained with either CD31 and CD34 (upper panels) or CD31 and CD43 (lower panels). A layer of the CD31-/CD34+ and/or CD31-/CD43+ cells emerged on the endothelial layer of the aorta of the wild-type and the heterologous Scl+/? E10.5 embryos, meanwhile several CD31+/CD34+ and/or CD31+/CD43+ cells are observed in the other lumens of these sections (A,B). On the other hand, a layer of CD31+ cells without CD34 or CD43 signals was shown in the E10.5 SCL?/? sections (C). Regardless of the genotypes, a few CD31+/CD34+ and/or CD31+/CD43+ cells were stained in the other cavities beyond the aorta (A–C).