Fig 1: SOX17 knockout impairs arterial specification and definitive hematopoiesis(A) Schematic diagram of hematopoietic development and in defined conditions. D, day of differentiation.(B) HB-CFC potential of wild-type or SOX17-/- H9 cells (means ± SDs, for 2 independent experiments performed in duplicate). **p < 0.01, t test.(C) Flow cytometric analysis of day 4 HE.(D and E) Flow cytometric analysis of day 5 HE. Graphs show the percentages and total number of cells generated from 104 hESCs (means ± SDs, n = 3 experiments). **p < 0.01 and ***p < 0.001, t test.(F) Schematic diagram of experiments. Wild and SOX17-/- cells were purified using CD31 magnetic-activated cell sorting (MACS) on day 4 and plated on OP9 or OP9-DLL4 for 5 days.(G) Hematopoietic colony-forming potential of day 4 HE after 5 days of culture on OP9 or OP9-DLL4 (means ± SDs, n = 2 experiments). ****p < 0.0001, 2-way ANOVA, Tukey’s multiple comparisons test.(H) Flow cytometric analysis of T cell differentiation.(I) Graphs show the total number of T cells generated from 104 CD43+ cells (means ± SDs, n = 3 experiments). ****p < 0.0001, 2-way ANOVA, Tukey’s multiple comparisons test.See also Figure S1.