Anti-PXN antibodies are used in the immunodetection of the protein paxillin. In humans, the canonical protein has a reported length of 591 amino acid residues and a mass of 64.5 kDa. Its subcellular localization is in the cytoplasm. Up to 4 different isoforms have been reported for this protein. It is notably widely expressed in many tissue types. A member of the Paxillin protein family, Pxn is known to be a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Post-translational modifications have been described, including phosphorylation. Synonyms for this target antigen include testicular tissue protein Li 134. PXN gene orthologs have been reported in the mouse, rat, bovine, frog, chimpanzee and chicken species. Over 500 citations in the literature describe the use of Pxn antibodies in research. Western Blot is a widely used application for these antibodies. ELISA, Immunofluorescence, and Immunohistochemistry are also common applications.