Genetex
Anti-Paxillin antibody
GTX125891
Polyclonal
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This rabbit polyclonal antibody directed against paxillin works well in immunofluorescence on PAN02 cells. The immunoreactivity was strong and specific for focal adhesions.
Immunofluorescence
PAN02 cells
1 hour in PBS 1% + normal goat serum +0.1% Triton X-100 at a dilution of 1:500
1 hour in PBS 1% + normal goat serum +0.1% Triton X-100
Goat anti-rabbit alexa 488 (Life Technologies) at a dilution of 1:500 for 1 hour
Not applicable
Epifluorescence microscopy, Nikon 100X objective
The rabbit polyclonal anti-paxillin antibody works well with standard immunofluorescence microscopy on PAN02 cells. The staining was strong and specific to predicted subcellular localization: focal adhesions. It is priced comparably to other vendors and antibodies.
N/A
Works well for IF microscopy
Have not tried it with other applications
Works well and priced accordingly