Anti-diphthamide biosynthesis 2 antibodies are used for the immunodetection of the protein encoded by the DPH2 gene. In humans, the canonical protein has a reported length of 489 amino acid residues and a mass of 52.1 kDa. Alternative splicing is reported to yield 3 different isoforms for this protein. It is reported to be highly expressed in skeletal muscle. A member of the DPH1/DPH2 protein family, it is known to be required for the first step of diphthamide biosynthesis, a post-translational modification of histidine which occurs in elongation factor 2. The gene encoding this protein is implicated in Developmental delay with short stature, dysmorphic facial features, and sparse hair. Other names for this target antigen include DPH2L2, 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2, DPH2 homolog, DPH2-like 2, and DEDSSH2. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species.