Anti-KRT1 antibodies are used in the immunodetection of the protein keratin 1. In humans, the canonical protein has a reported length of 644 amino acid residues and a mass of 66 kDa. Its subcellular localization is in the cell membrane and cytoplasm. It is notably expressed in the vagina, skin, oral mucosa, esophagus, and cervix. A member of the Intermediate filament protein family, KRT1 is believed to regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1).
The KRT1 marker can be used to identify Spinous Keratinocytes.* Synonyms for this target antigen include AEI2 and CK1. KRT1 gene orthologs have been reported in the mouse, rat and bovine species. Over 700 citations in the literature describe the use of KRT1 antibodies in research. Immunohistochemistry is a widely used application for these antibodies. Flow Cytometry, Western Blot, Immunocytochemistry, and Immunofluorescence are also common applications.
*HuBMAP Human Reference Atlas v1.4