Anti-HSF1 antibodies are used in the immunodetection of the protein heat shock transcription factor 1. In humans, the canonical protein has a reported length of 529 amino acid residues and a mass of 57.3 kDa. Its subcellular localization is in the nucleus and cytoplasm. Up to 2 different isoforms have been reported for this protein. It is notably widely expressed in many tissue types. A member of the HSF protein family, HSF1 is known to function as a stress-inducible and DNA-binding transcription factor that plays a central role in the transcriptional activation of the heat shock response (HSR), leading to the expression of a large class of molecular chaperones, heat shock proteins (HSPs), that protect cells from cellular insult damage. Synonyms for this target antigen include HSTF1 and heat shock factor protein 1. HSF1 gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Over 400 citations in the literature describe the use of HSF1 antibodies in research. Western Blot is a widely used application for these antibodies. ELISA, Immunocytochemistry, Immunofluorescence, and Immunohistochemistry are also common applications.