Anti-PDL1 antibodies are essential reagents in flow cytometry for detecting the target protein, programmed death-ligand 1. Encoded by the gene CD274 in humans, PD-L1 is the ligand for the inhibitory receptor PDCD1/PD-1, which modulates the activation threshold of T-cells and limits T-cell effector response. This interaction inhibits the cytotoxic T lymphocytes (CTLs) effector function. The protein is a 40 kDa transmembrane protein localized to the cell surface. Examine the supplier's provided validation data when comparing PDL1 antibodies for flow cytometry. Also look for application-specific recommendations, such as dilutions, protocols, sample types, and other helpful usage notes.