Cell lysates and extracts are commercially available from a number of manufacturers for use in biological research. Whole cell lysates are generally extracted from stable cell lines, whereas tissue lysates are collected from tissues or organs in human patient samples, animals, and even plants. Among their uses, lysates and extracts are useful as controls for protein-based applications, such as Western blotting and other immunoassays. Tissue lysates may also be useful materials in the study of various disease states. Browse our catalog to find lysate and extract products across different host species and compare between different suppliers. Lysates are generally partially purified, with cell debris removed leaving behind the total protein in liquid or lyophilized formats. However, it is recommended to refer to the supplier's descriptions for more information on the source, purification method, and intended applications.
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Upgrade your tissue culture room by getting accurate cell counts quickly, and recording results digitally. The Scepter™ 3.0 handheld cell counter combines smart capabilities with the Coulter impedance principal to hand you precise, consistent counts ...
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The MACSQuant® Tyto® Cell Sorter brings a gentler, safer, and sterile flow sorting technology to your lab. With the microchip technology at its heart, cells will no longer be exposed to electrical charge, decompression, and fluidic systems....
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The Promega CellTiter-Blue Cell Viability Assayis used for cell viability assay. The workflow is about as simple as it gets: add one reagent directly to cells growing in serum-containing medium, incubate 1–4 hours, and read fluorescence.
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I work on neonatal and adult mouse gut and routinely isolate immune cells from gut tissue. This requires tissue digestion and I often use Collagenase type VIII to dissociate tissue and isolate immune cells from lamina propria fraction.
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