Western Blot Imaging

Central to Western blot data analysis is imaging, which enables qualitative and quantitative measurement of specific protein expression. Western blot imaging equipment allow the researcher to visualize the processed blots by illuminating the membrane, recognizing signals over background noise, capturing the image, and analyzing relative intensities. While traditional Western blot processing on films has required the use of a dark room, many modern digital imaging systems are capable of directly imaging the membrane. Imagers used in Western blotting include gel documentation systems, which visualize acrylamide gels following protein separation. Imagers for chemiluminescent detection offer high sensitivity, signal stability, and ease of use. Multiplex fluorescence imaging systems, which often offer multiple modes of detection, are capable of imaging multiple unique signals simultaneously from a single experiment. Browse through a diverse selection of instruments from reliable manufacturers for publication-quality Western blot analysis.

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How to Select the Best Western Blot Detection Method

Western blot is considered to be among the simplest and most convenient techniques for identifying individual proteins within a complex mixture. Here we explain why this blotting type retains its appeal and provide some tips for selecting a suitable detection method.
Advances in Western Blot Data Imaging

When compared to film, digital detection benefits from a broader dynamic range, while making sharing results much easier—an especially important consideration when remote work is a necessity. In this article, we explain how digital imaging systems vary and comment on some recent advances that have increased their utility for western blotting.